畜牧兽医学报2017，Vol.48Issue(7)：1314-1322,9.DOI:10.11843/j.issn.0366-6964.2017.07.016
16S rRNA高通量测序方法检测羊圈空气微生物群落结构及多样性
Microbial Community Structure and Diversity of Sheepfold Atmosphere by 16S rRNA High-throughput Sequencing
摘要
Abstract
The aim of this study was to investigate the bacterial community structure and the characteristics of pathogenic bacteria in the air of sheepfold.We selected ten different sheep farms to take air samples.The Illumina MiSeq sequencing with 16S rRNA V3-V4 variable region was adopted to analyze the bacterial community structures of the air in sheepfold.A total number of 33 408 operational taxonomic units (OTUs) were obtained from air samples under the similarity level of 97％.We have got bacteria comprising groups of 25 phyla,66 classes,123 orders,253 families,547 genera,and 444 species.Analysis of microbial diversity and summarize pathogens with higher abundance in sheepfolds,which provides the basis for the disinfection and medication for sheep farm.The diversity,abundance,and characteristics of the main pathogens in air samples were analyzed in this study,which would be a helpful reference for disease prevention and control in sheepfold.关键词
羊圈空气/微生物群落及多样性/MiSeq测序Key words
air of sheep fold/microbial community structure and diversity/MiSeq sequencing分类
农业科技引用本文复制引用
孙翠丽,张阁,程汝佳,朱良全,丁家波,常维山..16S rRNA高通量测序方法检测羊圈空气微生物群落结构及多样性[J].畜牧兽医学报,2017,48(7):1314-1322,9.基金项目
国家重点研发计划(2016YFD0500902) （2016YFD0500902）
