畜牧兽医学报2017，Vol.48Issue(7)：1357-1364,8.DOI:10.11843/j.issn.0366-6964.2017.07.021
玉米赤霉烯酮对小鼠T淋巴细胞体外活化、增殖的影响
The Effects of Zearalenone on the Activation and Proliferation of Mouse T-lymphocytes in vitro
摘要
Abstract
In order to reveal the mechanism of the immunosuppressive effect of Fusarium toxin zearalenone (ZEA),this experiment had studied the effects of ZEA on the activation and proliferation of mouse T lymphocytes stimulated by concanavalin A (Con A) in vitro.After being treated with different concentrations of ZEA (0,10,20,40μmol · L-1),CCK-8 method was used to detect the proliferation of T lymphocytes.At the same time,the expression levels of the early activation antigen CD69 and metaphase activation antigen CD25 were evaluated by flow cytometry.Results were as follows:Cells were cultured for 48,72,96 h,compared with group of cell blank,the group of Con A had obvious proliferating phenomenon (P＜0.01).Compared with the group of Con A only,group of ZEA 10 μmoL · L-1 had an obvious phenomenon of Inhibition of proliferation (P＜0.05).When ZEA concentration was 20,40μmol · L-1,the proliferation of T lymphocytes was further inhibited.The proliferation of T lymphocytes stimulated by Con A decreased obviously with the increased concentration of the ZEA which showed in a dose-dependent manner (P＜0.01).Cells were stimulated by Con A for 6 or 30 hours.Compared with group of cell blank,the group of Con A had obvious activated phenomenon (P＜0.01).Compared with the group of Con A only,group of ZEA 10 μmol · L-1 had an obvious phenomenon of Inhibition of activation (P＜0.01).When ZEA concentration was 20,40 μmol · L-1,the expression of CD69 and CD25 was further inhibited.The expression levels of the early activation antigen CD69 and metaphase activation antigen CD25 were significantly inhibited after being treated with the ZEA which showed in a dose-dependent manner (P＜0.01).The immunosuppressive effect of ZEA to animals is related to its directly inhibition to the activation and proliferation of T lymphocytes in mouse.关键词
玉米赤霉烯酮/T淋巴细胞增殖/T淋巴细胞活化/Con A/CCK-8/流式细胞术/CD69/CD25Key words
zearalenone/T lymphocyte proliferation/T lymphocyte activation/Con A/CCK-8/flow cytometry/CD69/CD25分类
农业科技引用本文复制引用
蔡国栋,卞建春,孙凯,项自来,王玲,邹辉,顾建红,袁燕,刘学忠,刘宗平..玉米赤霉烯酮对小鼠T淋巴细胞体外活化、增殖的影响[J].畜牧兽医学报,2017,48(7):1357-1364,8.基金项目
国家重点研发计划支持项目(2016YFD0501208) （2016YFD0501208）
江苏省普通高校自然科学基金(08KJD230002) （08KJD230002）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
