烟草科技2017，Vol.50Issue(7)：1-6,13,7.
烟草同源异型盒转录因子NtKNATM1基因的克隆及表达分析
Cloning and expression analysis of transcription factor gene NtKNATM1 from Nicotiana tabacum
摘要
Abstract
KNOX (KNOTTED-like homeobox) genes encode a class of homeodomain transcription factors, which play important roles in shoot meristem initiation and maintenance and lateral meristem morphogenesis. To reveal the functions of KNOX in tobacco, a KNATM1 gene was cloned from Nicotiana tabacum by homology search. The open reading frame of NtKNATM1 was 522 bp in length and encoded 174 amino acids. Bioinformatics analysis indicated that NtKNATM1 encoded a KNOX protein with an atypical domain, which contained KNOX1 and KNOX2 domains. Phylogenetic analysis showed that NtKNATM1 was close to Arabidopsis thaliana KNATM in evolutionary distance, and these genes formed a unique KNOX cluster. qPCR showed that NtKNATM1 was highly expressed in axillary bud and terminal bud. Auxin significantly up-regulated the expression level of NtKNATM1, in comparison with the down-regulation effect of topping. Therefore, NtKNATM1 might involve in the developments of shoot meristem and lateral meristem, and be positively regulated by auxin.关键词
烟草/NtKNATM1/克隆/表达/分生组织/激素Key words
Nicotiana tabacum/NtKNATM1/Clone/Expression/Meristem/Phytohormone分类
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谢小东,王晨,罗朝鹏,魏攀,张剑锋,武明珠,李锋,王中,杨军..烟草同源异型盒转录因子NtKNATM1基因的克隆及表达分析[J].烟草科技,2017,50(7):1-6,13,7.基金项目
中国烟草总公司烟草基因组计划重大专项项目"烟草转录因子基因芯片研制及重要转录因子表达调控研究"[110201401011(JY-11)] （JY-11）
河南省博士后资助项目"烟草腋芽发育相关基因的克隆与功能研究"(2014010). （2014010）
