中国免疫学杂志2017，Vol.33Issue(6)：838-843,6.DOI:10.3969/j.issn.1000-484X.2017.06.007
β-胡萝卜素对脂多糖刺激巨噬细胞RAW264.7炎症因子的影响及其机制
Effects and mechanism of β-carotene on inflammatory factors in LPS-induced RAW264.7 cells
摘要
Abstract
Objective:To investigate the effects and mechanism of β-carotene on inflammatory factors (IL-1 β,IL-6,TNF-α) in LPS-induced RAW264.7 cells.Methods:Firstly,RAW264.7 cells of being induced by 4 (5 μg/ml)for 24 h were treated with different concentration of β-carotene (20,40,80,160 pmol/L)for 3 h.The cells viability was measured by MTIT,the mRNA relative expression of IL-1 β,IL-6,TNF-cα was detected by fluorescence quantitative PCR,the secretion capacity of IL-1 β,IL-6,TNF-α was detected by ELISA and the protein relative expression of NF-κB p65 protein was measured by Western blot.Secondly,RAW264.7 cells were induced by LPS(5 μg/ml) and different concentration of PDTC(1,5,10 μg/ml)for 24 h,NF-κB p65 protein was measured by Western blot and inflammatory factors were detected by fluorescence quantitative PCR and ELISA.Finally,compared the changes in the relative expression of inflammatory factors and NF-κB p65 protein between LPS+PDTC group and LPS+PDTC + β-carotene group.Results:Compared with the LPS-induced group,β-carotene could increase the cell viability of LPS-induced RAW264.7 cells and inhibied the relative expression of inflammatory factors and NF-κB p65 protein.Inhibited the relative expression of NF-κB p65 protein could reduce the relative expression of inflammatory factors.Compared with the LPS+PDTC group,LPS +PDTC + β-carotene group could inhibit the relative expression of inflammatory factors significantly (P＜0.05).But,there was little difference about the relative expression of NF-κB p65 protein between this two groups.Conclusion:β-carotene inhibits the relative expression of inflammatory factors(IL-1 β,IL-6,TNF-α) in LPS-induced RAW264.7 cells through inhibition of NF-κB p65 protein in NF-κB pathway,this pathway isn't unique.关键词
β-胡萝卜素/RAW264.7细胞/炎症因子/NF-κB p65蛋白Key words
β-carotene/RAW264.7 cell/Inflammatory factor/NF-κB p65 protein分类
农业科技引用本文复制引用
张晓音,张珊珊,吴旻,吉昱斌,黄羽盛,郑鑫..β-胡萝卜素对脂多糖刺激巨噬细胞RAW264.7炎症因子的影响及其机制[J].中国免疫学杂志,2017,33(6):838-843,6.基金项目
本文为吉林省科技厅科技成果转化促进计划(20150307021NY). （20150307021NY）
