中国麻业科学2017，Vol.39Issue(3)：111-119,9.
苎麻实时定量PCR体系的建立
Establishment of Real-time Quantitative PCR System for Ramie
摘要
Abstract
The application of real-time quantitative PCR technology is widely used at present.The accuracy of real-time quantitative PCR is affected by RNA quality and reverse transcription efficiency,amplification efficiency and reference gene.In this study,real-time quantitative PCR system was estab lished using 3 ramie varieties,2 ramie gene sequence,and actin gene sequence as the reference gene.The results showed that RNA extracted by RNAkits can meet the requirements of RT-qPCR.The ampli fication curve and melting curve were consistent with the experimental requirements.Actin gene can be used as a reference for research on relative quantitative analysis of ramie.Relative quantitative analysis shows that the RT-qPCR method established in this study can be used to study gene expression level.This study can provide reference for the application of real-time quantitative PCR in ramie.关键词
苎麻/实时定量PCR/RNAKey words
ramie/real-time PCR/RNA分类
农业科技引用本文复制引用
马鑫,谭松林,邢虎成,廖少波..苎麻实时定量PCR体系的建立[J].中国麻业科学,2017,39(3):111-119,9.基金项目
湖南省自然科学基金项目(2015JJ2083) （2015JJ2083）
