中国实用内科杂志2017，Vol.37Issue(7)：617-620,4.DOI:10.19538/j.nk2017070111
GAD65反应性T细胞检测在成人隐匿性自身免疫糖尿病筛查中的应用研究
Applications of the detection of GAD65 reactive T cells in latent autoimmune diabetes in adult
摘要
Abstract
Objective To explore the application of GAD65 reactive T cells(GAD65-T) detected by enzyme linked immune spot (ELISPOT) in screening LADA patients.Methods Totally 28 LADA patients,40 T2DM(Type 2 diabetes mellitus) patients and 20 healthy controls were recruited.GAD65-Ab were determined by radioligand assays.Human peripheral blood mononuclear cells were isolated by Ficoll-Hypaque density gradients.ELISPOT assay was performed to measure the number of spots-forming plaque.One spot-forming represents a GAD65-T cells.The clinical characteristics of two groups(ELISPOT positive or negative) were compared by ELISPOT in 40 T2DM patients.Results Compared with T2DM and control group,the number of GAD65-T cell significantly increased in LADA group (P＜0.01),there being significantly more GAD65-T cells in T2DM than in control group(P＜0.05).A positive response was defined when the cell number ＞ 95％CI of control subjects.The positive rate was 71.4％ (20 of 28) in LADA group,37.5(14 of 40) in T2DM group and 0 in control group.In 40 T2DM patients,the patients in ELISPOT-positive subgroup had lower BMI,higher HbA1c and worse β cells function compared with the negative subgroup.Conclusions GAD65-T cells are found in some patients initially diagnosed with T2DM(GAD65-Ab negative),combination of enzyme linked immune spot and GAD65-Ab assay can improve the detection rate of LADA.关键词
糖尿病/自身免疫/隐匿性/谷氨酸脱羧酶/T淋巴细胞/酶联免疫斑点Key words
diabetes/autoimmune/latent/glutamate decarboxylase/T-lymphocytes/enzyme linked immune spot assay分类
医药卫生引用本文复制引用
王婉茹,余靖,孙志纯,王爱红,洪真真,张翼,周智广..GAD65反应性T细胞检测在成人隐匿性自身免疫糖尿病筛查中的应用研究[J].中国实用内科杂志,2017,37(7):617-620,4.基金项目
福建省自然科学基金项目计划(2015J01517) （2015J01517）
泉州市科技计划项目(2016Z030) （2016Z030）
