中国现代医学杂志2017，Vol.27Issue(13)：1-5,5.DOI:10.3969/j.issn.1005-8982.2017.13.001
壳聚糖载体加载碱性成纤维细胞因子诱导成年大鼠骨髓间充质干细胞分化为神经细胞的研究
Differentiation of bone marrow mesenchymal stem cells of adult rat into nerve cells induced by chitosan carrier loaded with bFGF
摘要
Abstract
Objective To induce in vitro differentiation of bone marrow mesenchymal stem cells into neuron-like cells by biological material.Methods Using bone marrow adherent characteristic,bone marrow mesenchymal stem cells were extracted.Then the cells were identified by cell surface antigens CD45 and CD90.The cells were divided into three groups namely pure chitosan,chitosan-loaded bFGF group and bFGF group.The expressions of neural stem cell marker Nestin and neuron-like cell marker Tuj-1 were evaluated using immunocytochemical method on the 3rd and 7th d respectively.Results The proportion of Nestin-positive cells in the bFGF loaded group on the 3rd day was significantly higher than that in the other two groups (P ＜ 0.05).Similarly,the proportion of Nestin and Tuj1 double positive cells in the bFGF loaded group was significantly higher than that in the other two groups on the 7th day of induction (P ＜ 0.05).Conclusions bFGF-loaded chitosan can induce differentiation of bone marrow mesenchymal stem cells of adult rat into neuron-like cells at a high proportion,which provides a theoretical and experimental evidence for autologous transplantation in the treatment of neurodegenerative diseases and central nervous system injury.关键词
壳聚糖/碱性成纤维细胞因子/骨髓间充质干细胞/神经分化Key words
chitosan/bFGF/BMSC/neuronal differentiation分类
医药卫生引用本文复制引用
李曼丽,杨朝阳,李晓光..壳聚糖载体加载碱性成纤维细胞因子诱导成年大鼠骨髓间充质干细胞分化为神经细胞的研究[J].中国现代医学杂志,2017,27(13):1-5,5.基金项目
国家国际科技合作专项项目(No:2014DFA30640) （No:2014DFA30640）
