中国现代医学杂志2017，Vol.27Issue(13)：6-13,8.DOI:10.3969/j.issn.1005-8982.2017.13.002
肝纤溶颗粒与复方鳖甲软肝片对大鼠肝纤维化模型细胞凋亡及内质网应激相关因子表达影响研究
Effect of Hepatic Fibrinolysis Granules combined with Fufang Biejia Ruangan Tablets on apoptosis and endoplasmic reticulum related factor expressions in rat hepatic fibrosis model
摘要
Abstract
Objective To investigate the effects of Hepatic Fibrinolysis Granules combined with Fufang Biejia Ruangan Tablets on cell apoptosis and endoplasmic reticulum (ER) related factor expressions in rat hepatic fibrosis model.Methods A total of 48 male SD rats of clean grade were randomly divided into normal control group,model control group,Biejia Ruangan Tablets group and Hepatic Fibrinolysis Granules group with 12 rats in each group.The hepatic fibrosis models were copied in all the groups except the normal control group.After the model was made for 3 weeks,the rats were given medicines at the same time.In the normal control group and the model control group the rats were given intragastric administration of 1.5 ml/kg normal saline,once a day.In the Fufang Biejia Ruangan Tablets group the rats were given 33.3％ Fufang Biejia Ruangan Tablets solution 1.5 ml/kg for gavage,once a day.In the Hepatic Fibrinolysis Granules group the rats were given 33.3％ Hepatic Fibrinolysis solution 1.5 ml/kg for gavage,once a day.The medication continued for 6 weeks.In the 3rd,6th and 9th week of the experiment,the liver pathology was observed,the number of apoptosis cells and the levels of hepatic fibrosis indexes and ER-related indexes were detected in all the groups.Results Compared with other groups,the fibrosis grade score significantly decreased (P ＜0.05);the number of apoptotic hepatocytes was significantly reduced (P ＜ 0.05);the levels of hyaluronic acid,laminin,serum type Ⅲ procollagen and type Ⅳ collagen were decreased significantly (P ＜ 0.05);CHOP and ATF6 expression levels also decreased significantly (P ＜ 0.05) in the Hepatic Fibrinolysis Granules group.Conclusions Hepatic Fibrinolysis Granules decrease the number of apoptosis cells and hepatic fibrosis level in rats with hepatic fibrosis.They are better than Biejia Ruangan Tablets in improvement of the pathological changes of liver and adjustment of endoplasmic reticulum related indicators,therefore have clinical significance.关键词
肝纤溶颗粒/复方鳖甲软肝片/大鼠肝纤维化模型/细胞凋亡/内质网应激相关因子Key words
Hepatic Fibrinolysis Granules/Biejia Ruangan Tablets/rat model of hepatic fibrosis/cell apoptosis/endoplasmic reticulum related factor分类
医药卫生引用本文复制引用
钟军华,袁勇,宋飞飞,杨世忠,林丹..肝纤溶颗粒与复方鳖甲软肝片对大鼠肝纤维化模型细胞凋亡及内质网应激相关因子表达影响研究[J].中国现代医学杂志,2017,27(13):6-13,8.基金项目
海南省卫计委科学课题(琼卫2011-54) （琼卫2011-54）
