中国现代医学杂志2017，Vol.27Issue(13)：24-27,4.DOI:10.3969/j.issn.1005-8982.2017.13.005
阻塞性睡眠呼吸暂停综合征模型大鼠心肌氧化应激的损伤及复氧后的变化
Myocardial oxidative stress injury and changes after reoxygenation in rat model of obstructive sleep apnea syndrome
田培燕 1陈应康 2谢福珊 1杨顺茂3
作者信息
- 1. 黔南民族医学高等专科学校生理教研室 贵州黔南558000
- 2. 黔南民族医学高等专科学校病理学教研室 贵州黔南558000
- 3. 贵州医科大学第三附属医院老年科 贵州黔南558099
- 折叠
摘要
Abstract
Objective To establish obstructive sleep apnea syndrome (OSAHS) rat model by intermittent hypoxia (IH) and to observe the myocardial oxidative stress injury and changes after reoxygenation.Methods In this study,10 untreated rats were enrolled into control group (group A).Through 30-d IH,obstructive sleep apnea syndrome model was established in 30 rats.among which 10 rats had examination of venous blood and myocardial tissues just after model establishment (group B),10 rats had reoxygenation immediately after model establishment (group C) and their venous blood and myocardial tissues were examined after 30-d normal feeding,still 10 rats had IH for additional 30 days (group D) before examination of their venous blood and myocardial tissues.The content of serum malondialdehyde (MDA),the activity of serum superoxide dismutase (SOD),and the activity of glutathione (GSH) in myocardial tissue were detected and compared between the groups.Results Compared with the group A,the serum MDA content increased,the serum SOD activity and the activity of glutathione (GSH) in myocardial tissue decreased in the group B (P ＜ 0.05);the indexes returned to normal in the group C (P ＞ 0.05);however,in the group D,the myocardial GSH activity and the serum SOD activity were significantly decreased,and the serum MDA content was significantly increased (P ＜ 0.05).Conclusions Oxidative stress appears in OSAHS,and causes severe injury of myocardial tissue.Hypoxia can change MDA content,and SOD and GSH activity which will recover after reoxygenation.Hence,serum MDA content and SOD and GSH activity can reflect the severity of oxidative stress in myocardial tissues.关键词
阻塞性睡眠呼吸暂停综合征/间歇缺氧/持续缺氧/心肌氧化应激Key words
obstructive sleep apnea syndrome/intermittent hypoxia/rat/myocardial oxidative stress injury分类
医药卫生引用本文复制引用
田培燕,陈应康,谢福珊,杨顺茂..阻塞性睡眠呼吸暂停综合征模型大鼠心肌氧化应激的损伤及复氧后的变化[J].中国现代医学杂志,2017,27(13):24-27,4.
