中国现代医学杂志2017，Vol.27Issue(13)：28-32,5.DOI:10.3969/j.issn.1005-8982.2017.13.006
亚麻醉、镇静及麻醉剂量丙泊酚对高脂血症大鼠空间记忆及海马组织的影响
Influence of subanesthesia, sedation and anesthesia doses of Propofol on spatial memory and hippocampal tissues in rats with hyperlipidemia
李繁 1黎仕焕 1黄奕弟2
作者信息
- 1. 海南医学院第二附属医院麻醉科,海南海口571100
- 2. 海南医学院第一附属医院解剖教研室,海南海口570102
- 折叠
摘要
Abstract
Objective To discuss the influence of subanesthesia,sedation and anesthesia doses of Propofol on learning and memory functions,hippocampal tissues in rats with hyperlipidemia.Methods Totally 65 healthy adult male SD rats were randomly divided into basic diet group (15 rats) and high-fat diet group (50 rats).After 6 weeks of feeding,10 rats were used for measurement of blood lipids.The 40 rats of successful model were randomly divided into 4 groups.Group A had intraperitoneal injection of saline 75 mg/kg;groups B,C and D were anesthetized by intraperitoneal injection of subanesthetic (10 mg/kg),sedation (30 mg/kg) and anesthetic (375 mg/kg)doses of 1％ Propofol for 5 consecutive days.Morris water maze training was carried out and spatial memory was measured 20 min and 6-7 days after drug administration.On the 8th day after the first administration,the hippocampal tissues were obtained to measure the level of Tau protein phosphorylation,caspase-3 expression level and γ-amino butyric acid (GABA) level.Results With the increase of the days of drug administration and training,the escape latency was significantly shortened in the 4 groups (P ＜ 0.05).The escape latency in the groups C and D at different time points was significantly longer than that in the groups A and B (P ＜ 0.05),and the escape latency in the group D was significantly longer than that in the group C (P ＜ 0.05).In the search space experiments on the 7th day,the platform-crossing times of the groups C and D were significantly fewer than those of the group A and B (P ＜ 0.05),and the platform-crossing times of the group D were significantly fewer than those of the group C (P ＜ 0.05);the GABA content of the hippocampal tissues and the average optical density of positive cells were significantly higher in the groups C and D than in the groups A and B (P ＜ 0.05),and significantly higher in the group D than in the group C (P ＜ 0.05).Conclusions Sedation and anesthesia doses of Propofol can reduce the spatial memory in hyperlipidemia rats,which may be associated with high levels of GABA in hippocampus and Tau proteinhyperphosphorylation.关键词
丙泊酚/高脂血症/空间记忆/海马组织Key words
Propofol/hyperlipidemia/spatial memory/hippocampal tissue分类
医药卫生引用本文复制引用
李繁,黎仕焕,黄奕弟..亚麻醉、镇静及麻醉剂量丙泊酚对高脂血症大鼠空间记忆及海马组织的影响[J].中国现代医学杂志,2017,27(13):28-32,5.
