中国现代医学杂志2017，Vol.27Issue(13)：39-43,5.DOI:10.3969/j.issn.1005-8982.2017.13.008
5-氨基酮戊酸光动力疗法对瘢痕成纤维细胞的作用研究
Impact of ALA-PDT on fibroblasts from keloid and its mechanism
朱志娟 1王琪影 1王晓利 1宋凤敏1
作者信息
- 1. 郑州大学第一附属医院整形外科,河南郑州450052
- 折叠
摘要
Abstract
Objective To study the impact of 5-aminolevulinic acid based photodynamic therapy (ALA-PDT) on proliferation of fibroblasts from keloid and its mechanism for guiding the use of ALA-PDT treating keloid in clinic.Methods Fibroblasts from patients' keloid were primarily cultured,then those cells without significant difference in growth state were divided into control group,PBS group and 6 ALA groups.Each group received PDT of 635 nm light emitting diode (LED) laser irradiation.Cell counting kit-8 (CCK-8) was used to detect the suppressive rate of ALA-PDT on the proliferation of the fibroblasts.RT-PCR and Western blot were used to detect the mRNA and protein levels of Collagen Ⅲ.Results Compared with the control and PBS groups,ALA-PDT inhibited the proliferation of fibroblasts and the suppressive rate was gradually enhanced with the increase of the ALA concentration.When the concentration of ALA was 0.500 mmol/L,the inhibition was the strongest.The levels of Collagen Ⅲ mRNA and protein were decreased with the increase of the ALA concentration,and the levels of Collagen Ⅲ mRNA and protein were the lowest when the concentration of ALA was 0.500 mmol/L.Conclusions ALA-PDT could inhibit the proliferation of fibroblasts from keloid and the inhibition would be the strongest at the ALA concentration of 0.500 mmol/L.The mechanism may be associated with the suppression of the synthesis of Collagen Ⅲ in scar fibroblasts.关键词
5-氨基酮戊酸光动力疗法/瘢痕成纤维细胞/胶原蛋白ⅢKey words
ALA-PDT/fibroblast/keloid/CollagenⅢ分类
医药卫生引用本文复制引用
朱志娟,王琪影,王晓利,宋凤敏..5-氨基酮戊酸光动力疗法对瘢痕成纤维细胞的作用研究[J].中国现代医学杂志,2017,27(13):39-43,5.
