中国畜牧兽医2017，Vol.44Issue(7)：1967-1974,8.DOI:10.16431/j.cnki.1671-7236.2017.07.010
Bcl-xL及PTD-FNK蛋白的细胞保护作用研究进展
Research Progress on Cytoprotection of Bcl-xL and PTD-FNK Protein
摘要
Abstract
In this article,firstly,the structure and functions of Bcl-xL protein is briefly introduced.It is believed that Bcl-xL protein as a member of the Bcl-2 family,has the same head folding structures as Bcl-2 family,which plays an important role in suppressing apoptosis of cell and protecting various cells from injury.Then the author introduces the biological properties,functions, mechanisms and clinical application of PTD-FNK protein (a gain-of-function mutant of Bcl-xL).It is thought that PTD-FNK protein is able to rapidly penetrate into cell in vitro and in vivo, and can protect various types of cells from many damage through the inhibition of apoptosis in the path of mitochondrion,so being a potent protein with various therapeutic applications.Finally,the author points out that the difficulty of mass production and practical application of two proteins is that prokaryotic expression of the proteins is inclusion body,which need the complex refolding process in order to obtain soluble proteins,and proteins'' loss in the refolding process is great.The priorities and direction of future research are to realize the soluble expression and purify the two proteins in large quantities by optimizing expression conditions of induction,laying the foundation of their application to clinical medicine and cell preservation under room temperature, low temperature and frozen condition.关键词
Bcl-xL蛋白/PTD-FNK蛋白/细胞保护Key words
Bcl-xL protein/PTD-FNK protein/cytoprotection分类
生物科学引用本文复制引用
王英群,石博妹,李珣,许春荣,李美珍,唐荣福,王晓晔,胡传活..Bcl-xL及PTD-FNK蛋白的细胞保护作用研究进展[J].中国畜牧兽医,2017,44(7):1967-1974,8.基金项目
广西科学研究与技术开发计划项目(桂科能1598022-2) （桂科能1598022-2）
广西自然科学基金面上项目(2016GXNSFAA380024) （2016GXNSFAA380024）
广西畜禽品种改良站横向科技项目(20140220) （20140220）
