中国畜牧兽医2017，Vol.44Issue(7)：1975-1981,7.DOI:10.16431/j.cnki.1671-7236.2017.07.011
奶牛原代乳腺上皮细胞的培养及β酪蛋白mRNA的表达
Culture of Dairy Cow Primary Mammary Epithelial Cells and Expression of β-casein mRNA
摘要
Abstract
The aim of this study was to construct the culture method of primary mammary epithelial cells in vitro using mammary tissue and to test expression of β-casein mRNA in mammary gland epithelial cells.The fresh lactating mammary gland tissue was obtained, microscope observation was used for cell morphology and cell growth counting,and the Real-time PCR method was used for detection of β-casein mRNA expression.The results showed that purified primary mammary gland epithelial cells formed island-like and grew with typical shape of slabstone and cobblestone;Primary mammary gland epithelial cells growth curve was "S" type and accorded with rule of general cell growth.Mammary epithelial cells expressed β-casein mRNA successfully.In conclusion, the primary mammary epithelial cells with normal physiology function had been established successfully.The study provided favourable cell model for further research of the functions of mammary epithelial cells.关键词
奶牛/原代/乳腺上皮细胞Key words
dairy cow/primary/mammary epithelial cell分类
农业科技引用本文复制引用
蒲俊华,朱小瑞,绪欣,卢徐斌,毛永江,杨章平..奶牛原代乳腺上皮细胞的培养及β酪蛋白mRNA的表达[J].中国畜牧兽医,2017,44(7):1975-1981,7.基金项目
国家自然科学基金(31472067、31372286) （31472067、31372286）
