中国畜牧兽医2017，Vol.44Issue(7)：1982-1989,8.DOI:10.16431/j.cnki.1671-7236.2017.07.012
猪流行性腹泻病毒山西分离株S基因遗传变异分析
Genetic Variation Analysis of S Gene of Porcine Epidemic Diarrhea Virus Isolated in Shanxi Provine
摘要
Abstract
In order to investigate the variation in S gene of porcine epidemic diarrhea virus (PEDV), the 4 strains of PEDV S gene nucleotide sequences were obtained, through RT-PCR amplification of tissue samples from Shanxi province.The obtained sequences and the deduced amino acid were analyzed and compared with the other published PEDV strains.Sequence analysis showed that compared with CV777 vaccine, there were 12 nucleotides insertions between 170 to 171 bp, 3 nucleotides insertions between 401 to 402 and 454 to 455 bp, 6 nucleotides deletion between 461 to 468 bp.The nucleotide and amino acid homologies were 99.2% to 99.8% and 98.6% to 99.7% respectively among 4 strains of PEDV S gene;Comparing with the strains isolated from China in 2011 to 2015, CV777 vaccine, attenuated DR13 and CV777, the nucleotide homologies were 95.0% to 98.5%,93.2% to 93.6%,92.1% to 92.9%,93.7% to 94.4%,respectively.The amino acid homology were 96.2% to 98.9%,91.9% to 92.9%,91.9% to 92.6%,92.9% to 94.0%, respectively.Phylogenetic analysis revealed that 4 strains of PEDV S gene belonged to the first group and had high correlative genetic relationship with the PEDV strains which isolated after 2010 in China, and had far correlative genetic relationship with the PEDV strains which isolated before 2010 in China, 2 strains of Japanese, 7 strains of South Korea, 2 vaccine strains.The results suggested that the prevalence of PEDV in Shanxi province had a more obvious variation.Therefore, it was necessary to develop a new vaccine to control the outbreak of PEDV.关键词
猪流行性腹泻病毒/S基因/序列分析Key words
porcine epidemic diarrhea virus (PEDV)/S gene/sequence analysis分类
农业科技引用本文复制引用
刘文俊,李红丽,赵岳,姚敬明,吴忻,孟帆,韩一超,王娟萍,樊振华,薛翼鹏,米瑞娟..猪流行性腹泻病毒山西分离株S基因遗传变异分析[J].中国畜牧兽医,2017,44(7):1982-1989,8.基金项目
山西省科技攻关项目(猪流行性腹泻分子流行病学调研与防控技术研究20140311020-2-A) （猪流行性腹泻分子流行病学调研与防控技术研究20140311020-2-A）
山西省科技攻关项目(山西猪流行性腹泻病原研究及防控对策20150311018-3) （山西猪流行性腹泻病原研究及防控对策20150311018-3）
