中国畜牧兽医2017，Vol.44Issue(7)：2033-2041,9.DOI:10.16431/j.cnki.1671-7236.2017.07.019
孕鼠暴露双酚A对仔鼠存活率、生殖激素及相关基因的影响
Effects of Maternal Exposure to Bisphenol A on Survival Rate, Reproductive Hormones and Relative Genes of Offspring Mice
摘要
Abstract
This study was aimed to investigate the reproductive toxicity of bisphenol A (BPA) exposed to the mother on the offsprings mice.Forty pregnant Kunming mice were randomly divided into 4 groups, i.e.groups A, B, C and D with 10 mice in each group.Group A was the control group and the mice received conventional feeds, mice in groups B, C and D were given 50,500 and 2 500 mg/kg BW BPA in feedstuffs during the whole gestation period (from 1 d to parturition), respectively.The death rates of the offsprings were calculated every week.The offspring mice were sacrificed at 56 days of age (at puberty).The morphology of ovary and testicular tissues were observed with hematoxylin-eosin (HE) staining.The levels of estradiol (E2), follicle stimulating hormone (FSH), testosterone (T) in mice serum were detected with ELISA Kit.The protein levels of Bax and Bcl-2 in ovary or testicular tissues were detected with immunohistochemistry, and the StAR,CYP11a mRNA levels in testicular tissues, the AMH, Kitlg mRNA levels in ovary were measured using Real-time PCR.The results showed that exposure of BPA to the mother extremely significantly increased the mortality (P<0.01),and significantly reduced the testicular weight of offspring mice (P<0.05).Maternal exposure to BPA extremely significantly reduced the levels of T (♂) and FSH(♀) (P<0.01),and extremely significantly elevated E2 (♀) level in offspring mice (P<0.01).BPA exposure damaged the testicular with less leydig cells and ovarian tissues with more vacuoles and less corpus granules in offspring mice.Immunohistochemistry results revealed that maternal exposure to BPA increased the Bax protein level and decreased the Bcl-2 protein level of testicular and ovary tissues in offspring mice.BPA significantly reduced the StAR mRNA expression in male offsprings (P<0.05).However, the mRNA level of CYP11a in groups B and D extremely significantly decreased while group C showed an significant elevation in male offsprings (P<0.01).The expression levels of Kitlg mRNA in groups C and D were decreased extremely significantly in female offsprings (P<0.01), the AMH mRNA expression in groups C and D increased significantly (P<0.05).The conclusion indicated that pregnant mice exposed to different doses of BPA had harmful effects on survival rate in offspring mice, and impact the reproductive hormones, proteins and genes expression.关键词
双酚A/小鼠/生殖激素/免疫组织化学/生殖基因Key words
BPA/mice/reproductive hormone/immunohistochemistry/reproductive genes分类
生物科学引用本文复制引用
马双,宋鹏琰,翟福展,钟秀会..孕鼠暴露双酚A对仔鼠存活率、生殖激素及相关基因的影响[J].中国畜牧兽医,2017,44(7):2033-2041,9.基金项目
国家科技部十二五科技支撑计划课题"防治家畜胎产病和提高畜禽免疫力中兽药创制及应用示范"(2011BAD34B02) （2011BAD34B02）
