中国畜牧兽医2017，Vol.44Issue(7)：2042-2049,8.DOI:10.16431/j.cnki.1671-7236.2017.07.020
抗氧化剂对马精子低温及冷冻保存效果的影响
Effects of Antioxidants on the Quality of Hypothermic Preservation and Frozen-thawed Equine Semen
摘要
Abstract
This study was aimed to evaluate the effects of various antioxidants, namely glutamine (0.015 g/mL), glycine (0.019 g/mL), cysteine (0.024 g/mL), methionine (0.015 g/mL), taurine (0.063 g/mL), vitamin C (0.4 mg/mL), vitamin E (0.5 mg/mL) and melatonin (0.001 mg/mL) on equine sperm quality after chill or freeze-thaw.Semen were collected from 6 adult thoroughbred stallions, INRA82 was used as the base extender (control group), adding INRA82 with different antioxidants was used in experimental group.Assess the effect of antioxidants on semen by detecting motion parameters after storage at 5 ℃ for 48 h.Motion parameters, plasma membrane integrity (PMI) and mitochondrial membrane potential were used to evaluate semen quality after thawing.The extender supplemented with 25 mmol/L taurine led to higher TM and PM, and supplemented with 0.4 mg/mL vitamin C obtained significant higher PM compared with control group (P<0.05) after storage at 5 ℃ for 48 h.The freeze extender supplemented with 0.5 mg/mL vitamin E or 0.015 g/mL methionine significantly increased the mitochondrial membrane potential compare with control group (P<0.05).No significant differences were observed for PMI and acrosomes integrity rate after frozen-thawed (P>0.05), but there was a trend that PMI of adding methionine and glycine group was higher than control group.The results suggested that extender supplemented with taurine and vitamin C could improve the semen preservation effect, and the extender supplemented with methionine could improve plasma membrane integrity, mitochondrial membrane potential of thawing sperm, and also could prolong the survival time of frozen thawed sperm.关键词
抗氧化剂/精液/冷冻保存/马Key words
antioxidants/semen/cryopreservation/horse分类
农业科技引用本文复制引用
徐文慧,姚新奎,郑新宝,于维浩,孟军,罗永明,李海,曾亚琦,王建文,毋状元..抗氧化剂对马精子低温及冷冻保存效果的影响[J].中国畜牧兽医,2017,44(7):2042-2049,8.基金项目
国家自然科学基金:马精子冷冻保存方法及精子冻融损伤机制的研究(31360550) （31360550）
法国速步马及培育关键技术引进实验研究(2014DFA31370) （2014DFA31370）
