中国畜牧兽医2017，Vol.44Issue(7)：2126-2131,6.DOI:10.16431/j.cnki.1671-7236.2017.07.031
禽流感病毒与鸡细小病毒二重PCR检测方法的建立
Development of a Duplex PCR Assay for Detection of Avian Influenza Virus and Chicken Parvovirus
摘要
Abstract
In order to establish a method to simultaneously detect avian influenza virus (AIV) and chicken parvovirus (ChPV),two pairs of specific primers were designed according to the sequences of AIV M gene and ChPV NS gene in GenBank.The duplex PCR assay was established by optimizing the reaction conditions.The tests showed that this method had high specificity, could simultaneously detect AIV and ChPV and no specific band was amplified for other subtypes avian pathogenic virus.The sensitivity result showed that the lower detection limit of this method was 100 fg.The results of 159 clinical samples were consistent with the sequencing results of PCR positive product.The double PCR methods for detection of AIV and ChPV established in this study had the characteristics of good specificity and high sensitivity, which was of great significance to the prevention control of AIV and ChPV.关键词
禽流感病毒(AIV)/鸡细小病毒(ChPV)/二重PCR/检测Key words
avian influenza virus (AIV)/chicken parvovirus (ChPV)/duplex PCR/detection分类
农业科技引用本文复制引用
何莹,张艳芳,谢芝勋,罗思思,李孟,谢丽基,邓显文,谢志勤,奉彬,黄娇玲..禽流感病毒与鸡细小病毒二重PCR检测方法的建立[J].中国畜牧兽医,2017,44(7):2126-2131,6.基金项目
广西科技基地和人才专项项目(桂科AD16380009) （桂科AD16380009）
桂渔牧科(201204930、201452001) （201204930、201452001）
国家"万人计划"领军人才专项 (2016-37-88) （2016-37-88）
广西科技项目(15-140-36-A-1) （15-140-36-A-1）
