中国畜牧兽医2017，Vol.44Issue(7)：2203-2208,6.DOI:10.16431/j.cnki.1671-7236.2017.07.043
鸡β干扰素在毕赤酵母中的分泌表达及其抗病毒活性测定
Expression of Chicken Interferon-β in Pichia pastoris and Its Antiviral Effect
刘新文 1王秀丽 1郭东春 2郭伟伟 1邹敏 1范根成1
作者信息
- 1. 青岛易邦生物工程有限公司,青岛266032
- 2. 哈尔滨兽医研究所,哈尔滨150069
- 折叠
摘要
Abstract
In order to study the expression of chicken interferon-β (ChIFNβ) in Pichia pastoris and its antiviral effect,ChIFNβ gene was subcloned into pPICZα-A vector and constructed pPIC-ChIFNβ recombinant plasmid.After linearized by Sac Ⅰ, the plasmid was transferred into Pichia pastoris X-33 strain.The recombinant strain was induced by 1.0% methanol for 4 d,the product of recombinant ChIFNβ was detected by SDS-PAGE,the antiviral activity was determined by classical cell pathological effect inhibition assay.The protein weight was approximate 17 ku and the recombinant protein was secreted effectively in Pichia pastoris.Potency of interferon was 107.5 U/mL.The expressed protein could also inhibit H9N2 avian influenza virus proliferation in chick embryo.Animal experiment suggested that recombinant protein could provide 70% immune protection for aviadenovirus.The results showed that the recombinant ChIFNβ had good antivirus activity,it would be reference for clinical application of ChIFNβ.关键词
鸡β干扰素/分泌表达/抗病毒活性Key words
chicken interferon-β/secreted expression/antiviral activity分类
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刘新文,王秀丽,郭东春,郭伟伟,邹敏,范根成..鸡β干扰素在毕赤酵母中的分泌表达及其抗病毒活性测定[J].中国畜牧兽医,2017,44(7):2203-2208,6.
