中国畜牧杂志2017，Vol.53Issue(7)：18-24,7.DOI:10.19556/j.0258-7033.2017-07-018
绵羊Lpin3基因多态性及其与尾型和屠宰性状的关联研究
Polymorphisms of Lpin3 Gene and the Associations with Tail Type and Slaughter Traits of Sheep
摘要
Abstract
The purpose of this study was to evaluate the effects of genetic variations of Lpin3 gene on the meat quality traits of sheep.The single nucleotide polymorphisms (SNPs) of Lpin3 gene partial 5' non-coding region in two breeds of fat-tailed sheep such as Guangling Large Tailed and Small Tailed Han were detected using direct DNA sequencing,and the associations between SNPs and tail type and slaughter traits were analyzed.The results showed that 13 novel SNPs were discovered in 5' non-coding region about 1 200 bp upstream sequence from start codon ATG of Lpin3 gene,among the insertion mutation of-334～-332 ins TAA significantly decreased the tail width (P＜0.05),while increased the slaughter traits (P＞0.05) in Small Tailed Han sheep.Meanwhile,mutation frequency of-334～-332 ins TAA in Small Tailed Han sheep was significantly higher than that in Guangling Large Tailed sheep (P＜0.05).The insertion mutation didn't significantly affect the examined traits in Guangling Large Tailed sheep (P＞0.05).In spite of the non-significant difference,-443 T＞C had the similar genetic effects as those of-334～-332 ins TAA in Small Tailed Han sheep.This study would provide the theoretical basis for the gene function of Lpin3 as well as the marker-assisted selection (MAS) of the meat quality traits in sheep.关键词
绵羊/Lpin3基因/单核苷酸多态性/关联研究Key words
Sheep/Lpin3 gene/Single nucleotide polymorphisms/Association study分类
农业科技引用本文复制引用
焦小丽,景炅婕,乔利英,李留安,陈明明,田川尧,康翠翠,刘文忠..绵羊Lpin3基因多态性及其与尾型和屠宰性状的关联研究[J].中国畜牧杂志,2017,53(7):18-24,7.基金项目
国家自然科学基金(31372292) （31372292）
天津农学院科技发展基金(2013N01) （2013N01）
天津市高校创新团队建设项目(TD12-5019) （TD12-5019）
