中国畜牧杂志2017，Vol.53Issue(7)：25-28,4.DOI:10.19556/j.0258-7033.2017-07-025
MC4R在小鼠下丘脑的定位及其基因在发情周期下丘脑、卵巢中的表达变化
Localization and Expression of MC4R in Mouse Hypothalamus and Ovary during Estrous Cycle
摘要
Abstract
Melanocortin-4 receptor (MC4R) plays an important role in feeding behavior and body weight regulation,While its function in reproductive activities is not clear.So,we used C57 mice in different estrus cycles to locate MC4R by immunofluorescence technique.Western blot was used to determine the expression of melanocortin-4 receptor protein in the proestrus,estrus,metestrus and diestrus of mouse hypothalamus and ovary.The results showed,MC4R were widely distributed in the third ventricle of the hypothalamus.The expression of MC4R in the estrus of hypothalamus was significantly lower than that in the other three periods (P ＜0.05).Meanwhile,the expression of MC4R in the ovaries was significantly lower than that in the proestrus and diestrus (P ＜0.01),and the MC4R was significantly higher in the diestrus (P ＜0.01) than in the other three periods.The results showed that the changes of MC4R expression in hypothalamus and ovary tissue in different stages of estrus cycle,which would provide a theoretical basis for further study of MC4R in mouse reproductive activity.关键词
MC4R/发情周期/下丘脑/卵巢Key words
MC4R/Estrous Cycle/Hypothalamus/Ovary分类
农业科技引用本文复制引用
李婷,潘登,范奎奎,李海军,曹贵方,杜晨光..MC4R在小鼠下丘脑的定位及其基因在发情周期下丘脑、卵巢中的表达变化[J].中国畜牧杂志,2017,53(7):25-28,4.基金项目
国家自然科学基金(31160455) （31160455）
内蒙古自然科学基金(2014MS0323) （2014MS0323）
中国博士后科学基金(2013M541212) （2013M541212）
