中国畜牧杂志2017，Vol.53Issue(7)：35-39,5.DOI:10.19556/j.0258-7033.2017-07-035
乌珠穆沁羊不同组织基因组DNA甲基化状态的SAP分析
Analysis of DNA Methylation in Different Ujumqin Sheep Tissues with MSAP
摘要
Abstract
The CCGG sites in genomes were detected among heart,liver,spleen,lung,kidney,stomach and longissimus muscle of 5 Ujumqin sheep for 10 pairs of selective primers by methylation sensitive amplified polymorphism (MSAP) method.A total of 1249 DNA fragments were detected by the polyacrylamide gel electrophoresis,including 154 type Ⅱ and 231 type Ⅲ fragments.Through calculation the methylation rates of different tissues for Ujumqin sheep,the average methylation rate of heart,liver,spleen,lung,kidney,stomach and longissimus muscle was 37.58％,39.88％,50.63％,25.82％,20.53％,28.19％,19.21％ respectively.For the all detected tissues,the spleen tissue methylation degree was The highest and the muscle tissue was the lowest.This study laid the foundation for further understanding of the role of DNA methylation regulatory effect for the sheep tissue differentiation.关键词
乌珠穆沁羊/组织/甲基化/MSAPKey words
Ujumqin Sheep/Tissue/Methylation/MSAP分类
农业科技引用本文复制引用
何小龙,荣威恒,付绍印,李蓓,祁云霞,王标,特日格勒,郝晨芳,达赖,刘永斌..乌珠穆沁羊不同组织基因组DNA甲基化状态的SAP分析[J].中国畜牧杂志,2017,53(7):35-39,5.基金项目
内蒙古自治区农牧业科学院青年创新基金(2013QNJM01) （2013QNJM01）
国家肉羊产业技术体系(CARS-39) （CARS-39）
内蒙古自治区农牧业科学院创新基金(2015CXJJM02) （2015CXJJM02）
内蒙古自治区草原英才建设项目 （）
