中国畜牧杂志2017，Vol.53Issue(7)：40-43,52,5.DOI:10.19556/j.0258-7033.2017-07-040
IGF2、H-FABP和RPL9基因在豫南黑猪和三元杂交猪中的差异表达
Differentially Expressions of IGF2、 H-FABP and RPL9 Genes in Yunan Black Pig and Three Breed Hybrid Pigs
白鹭 1景亚星 1冯硕 1孙港 1郑振宇1
作者信息
- 1. 河南农业大学牧医工程学院,河南郑州450002
- 折叠
摘要
Abstract
This experiment was conducted to study the differentially expressions of IGF2,H-FABP and RPL9 genes in different tissues between the yunan black pig and the three breed hybrid pig and analyzed the differences of gene expression between different pigs.The yunan black pig and the" Duroc × large white × Landrace" three breed hybrid pig,weighing an average of 70 kg,were randomly chosen to obtain the heart,liver,spleen,lung,kidney,abdominal fat and back subcutaneous fat for total RNA extraction and fluorescence quantitative PCR test of the IGF2,H-FABP and RPL9 gene.The results showed that the expression differences ofIGF2 in heart,liver,spleen tissues of yunan black pig were especially obvious that it of the yunan black pig were about 225.0 times of the three breed hybrid pig (P＜0.05).The transcription levels of H-FABP in the yunan black pig heart,spleen,and subcutaneous tissues were higher than those in the three breed hybrid pig,which the expression in spleen tissue of the yunan black pig was about 6.8 times of the three breed hybrid pig (P＜0.05),while the expressions in lung and abdomen fat of the three breed hybrid pig were higher,that H-FABP expression in abdomen fat of the three breed hybrid pig was about 2.7 times of the yunan black pig (P＜0.05).The expressions of RPL9 in heart,liver,lung and kidney tissues of the yunan black pig were higher and the expression difference in subcutaneous fat was largest,which of.the three breed hybrid pig was about 4.9 times that of the yunan black pig (P＜0.05).These results indicated that the expression differences of the IGF2、H-FABP and RPL9 gene in different tissues between the yunan black pig and the three breed hybrid pig were significant.关键词
豫南黑猪/三元杂交猪/IGF2/H-FABP/RPL9/脂肪沉积Key words
Yunan black pig/Three breed hybrid pig/IGF2/H-FAB/PRL9/Fat deposition分类
农业科技引用本文复制引用
白鹭,景亚星,冯硕,孙港,郑振宇..IGF2、H-FABP和RPL9基因在豫南黑猪和三元杂交猪中的差异表达[J].中国畜牧杂志,2017,53(7):40-43,52,5.
