中国畜牧杂志2017，Vol.53Issue(7)：48-52,5.DOI:10.19556/j.0258-7033.2017-07-048
黑萨福克和杜泊羊新鲜和冷冻胚胎移植效率的研究
Study of Fresh and Frozen Embryo Transfer Efficiency of Black Suffolk and Dorper Ewes
摘要
Abstract
To improve the embryo transfer efficiency of elite species of sheep with high meat quality,a total of 76 Black Suffolk and 27 Dorper ewes were treated by "CIDR+FSH+PMSG" method for superovulation as donors.The 384 small tailed han sheep were treated by "CIDR+PMSG" method for estrus synchronization as receptors.It was explored to test the influence of developmental stages of embryo,frozen processing of the embryo,embryo in vitro residence time and transplantation of lateral corpus luteum number of receptor on the pregnancy rates after embryo transfer.The pregnancy rate of blastocyst transplantation was significantly higher than morula transplantation (P＜0.01) when a single fresh embryo was transplanted.The frozen/thaw blastocyst transplantation pregnancy rate was significantly lower than fresh blastocyst (P＜0.01),but the difference between frozen/thaw morula and fresh morula was not significant(P＞0.05).In addition,the pregnancy rate with transplanted morula would be significantly affected (P＜0.01),the transplanted blastocyst would not be significantly affected(P＞0.05),when the transplanted-side-ovary of receptor had 1 corpus luteum or 2～3 corpus luteums.In conclusion,when morula and blastocyst were tansplanted into uterine horn,higher pregnancy rate could be achieved if a fresh blastocyst or a frozen morula was transfered.Fresh morula should be transplanted to the receptor with 2～3 corpus luteums on ovary transplantation side instead of 1 corpus luteum.关键词
绵羊/桑椹胚/囊胚/冷冻胚胎/受体黄体数/妊娠率Key words
Sheep/Morula/Blastocyst/Frozen embryo/Corpus luteum number of receptor/Pregnancy rate分类
农业科技引用本文复制引用
徐志远,杨明辉,姬鹏云,张效生,张金龙,朱士恩,阿布力孜·吾斯曼,赵静,刘国世..黑萨福克和杜泊羊新鲜和冷冻胚胎移植效率的研究[J].中国畜牧杂志,2017,53(7):48-52,5.基金项目
转基因重大专项(2014ZX08008-002B、2016ZX08008-003) （2014ZX08008-002B、2016ZX08008-003）
新疆自治区科技支疆项目(2016E02037) （2016E02037）
中国农业大学与新疆农业大学合作基金(2015TC050) （2015TC050）
