中国畜牧杂志2017，Vol.53Issue(7)：56-61,6.DOI:10.19556/j.0258-7033.2017-07-056
油酸对奶牛乳腺上皮细胞内甘油三酯含量和乳脂肪合成相关基因表达的影响
Effects of Oleic Acid on Triglyceride Content and Gene Expressions Involved in Milk Fat Synthesis in Bovine Mammary Epithelial Cells
摘要
Abstract
The aim of this study was to determine the effects of oleic acid on triglyceride content and gene expressions involved in milk fat synthesis in bovine mammary epithelial cells (BMECs).Mammary epithelial cells from Chinese Holstein cows were culture and purification.Different concentrations [0(control),50,100,200,400 μtmol/L] of oleic acid were added in culture medium for a 24 h cultivation.Cell viability was detected by thiazoly blue tetrazolium bramide (MTT);triglyceride (TAG) content was measured by using TAG determination kit;gene expressions involved in milk fat synthesis were measured by real-time quantitative (RT-qPCR).The results showed that 200 μmol/L and 400 μmol/L oleic acid significantly inhibited the proliferation of BMECs compared with control and other experimental groups (P＜0.05);100 μmol/L and 200 μmol/L oleic acid significantly promoted TAG synthesis in BMECs (P＜0.05).SCD,FABP3 and SREBF1 gene expression were significantly suppressed by addition of oleic acid (P＜0.05),however,DGAT2 gene expression was increased.50 μ mol/L oleic acid significantly increased ACC and PPARGγ gene expression(P＜0.05).In conclusion,50 to 100 μmol/L oleic acid is an optimal level considering its improvement effects on milk fat synthesis.关键词
奶牛乳腺上皮细胞/油酸/细胞活力/甘油三酯/乳脂肪合成相关基因Key words
Bovine mammary epithelial cells/Oleic acid/Cell viability/Triglyceride/Genes related in milk fat synthesis分类
农业科技引用本文复制引用
邢媛媛,李红磊,李大彪,胡红莲,张兴夫,高民..油酸对奶牛乳腺上皮细胞内甘油三酯含量和乳脂肪合成相关基因表达的影响[J].中国畜牧杂志,2017,53(7):56-61,6.基金项目
现代农业(奶牛)产业技术体系建设专项资金(CARS-37) （奶牛）
