中国药房2017，Vol.28Issue(21)：2978-2980,3.DOI:10.6039/j.issn.1001-0408.2017.21.27
葛花配方颗粒的UPLC指纹图谱研究
Study on UPLC Fingerprint of Gehua Formula Granules
摘要
Abstract
OBJECTIVE:To establish UPLC fingerprint of Gehua formula granules. METHODS:UPLC method were adopted. The determination was performed on Zorbax Eclipse XDB-C18 column with mobile phase consisted of acetonitrile-water at the flow rate of 0.5 mL/min. The detection wavelength was set at 264 nm,column temperature was 25 ℃,and the sample size was 1 μL. Using tectorigenin as reference substance,UPLC chromatograms of 10 batches of Gehua formula granules were determined. The common peak identification and similarity evaluation were conducted by TCM Chromatogram Fingerprint Similarity Evaluation Sys-tem(2004 A edition). RESULTS:14 common peaks were identified in UPLC chromatograms of 10 batches of Gehua formula gran-ules and similarities were all higher than 0.90. UPLC chromatograms of 10 batches of samples were in good agreement with control fingerprint. CONCLUSIONS:Established UPLC fingerprint can provide reference for identification and quality evaluation of Gehua formula granules.关键词
葛花配方颗粒/超高液相色谱法/指纹图谱/鸢尾苷元Key words
Gehua formula granules/UPLC/Fingerprint/Tectorigenin分类
医药卫生引用本文复制引用
刘梦云,江洁怡,毕晓黎,李素梅,李养学..葛花配方颗粒的UPLC指纹图谱研究[J].中国药房,2017,28(21):2978-2980,3.基金项目
广东省省级科技计划项目(No.2015B070701010) （No.2015B070701010）
