中国油料作物学报2017，Vol.39Issue(3)：334-341,8.DOI:10.7505/j.issn.1007-9084.2017.03.007
基于SLAF-seq技术构建亚麻高密度遗传图谱
High density genetic map of flax based on SLAF-seq technology
摘要
Abstract
To develop high-throughput SNP markers for high quality map of flax (Linum usitatissimum L.),100 individuals of 2 parents and their F2 population were detected by SLAF-seq technology.They were identified by high-throughput sequencing for SNP marker development and high-density genetic mapping.The 2 parents were varieties R43 and LH-89.After in silico digestion of reference genome,2 enzymes Hae Ⅲ and Rsa Ⅰ were used to digest the genome DNA.Restriction fragments of 314-414bp were defined as SLAF.Polymorphic SLAFs were analysed and SNPs were detected according to data obtained by high-throughput sequencing.A high-density genetic map was constructed using HighMap software.196.29M reads were obtained,with average Q30 value of 81.95％,and average GC content 38.64％.260 380 SNPs were detected,of which 4 547 SNPs were qualified for genetic map construction.The final flax genetic map included 4 145 SNP markers on 15 linkage groups and was 2 632.94 cM in length with an average distance of 0.96 cM between adjacent markers.This first high density genetic map in flax would be essential for genetic study and marker assistant breeding of flax.关键词
亚麻/SLAF-seq技术/高通量测序/SNP标记/高密度遗传图谱Key words
flax/SLAF-seq technology/high-throughput sequencing/SNP markers/high-density genetic map分类
农业科技引用本文复制引用
高凤云,斯钦巴特尔,张辉,贾霄云,伊六喜,周宇,李强,叶应福,侯建华..基于SLAF-seq技术构建亚麻高密度遗传图谱[J].中国油料作物学报,2017,39(3):334-341,8.基金项目
内蒙古农牧业创新基金项目(2011CXJJM01-2) （2011CXJJM01-2）
国家胡麻产业技术体系(CARS-17-GW-3) （CARS-17-GW-3）
内蒙古自治区创新人才团队项目 （）
