中国油料作物学报2017，Vol.39Issue(3)：386-392,7.DOI:10.7505/j.issn.1007-9084.2017.03.014
花生疮痂病病原菌分离技术研究
A technique for isolation of Sphaceloma arachidis from infected peanut
摘要
Abstract
The causal agent of peanut scab disease is difficult to isolate due to its extremely slow growth and difficult to sporulate on agar media.In this study,an efficient technique for tissue isolation and single-spore isolation of the pathogene was established.Results showed that pathogen isolation frequency from leaves was significantly higher than that of stems.The optimum surface-sterilizing time was 1-3 min,sampling time and storage time within 48h after sampling had no significant influences on isolation frequency.Abundant single-spore colonies could be obtained by improved dilution-plate method,the number of single-spore colonies on peanut leaf decoction agar (PLDA) was more than that on PDA medium,PLDA could inhibit undesired microbes significantly.The number of single-spore colonies obtained by dilution-plate method at early and middle stage of infection was significantly greater than that of late stage,and few single-spore colonies were obtained at the late stage.关键词
花生疮痂病/Sphaceloma arachidis/分离技术/单孢分离/组织分离Key words
peanut scab disease/Sphaceloma arachidis/isolation technique/single-spore isolation/tissue isolation分类
农业科技引用本文复制引用
薛彩云,傅俊范,周如军,崔建潮,徐喆,赵杰锋,李元杰..花生疮痂病病原菌分离技术研究[J].中国油料作物学报,2017,39(3):386-392,7.基金项目
辽宁省农业攻关计划(2011214002) （2011214002）
辽宁省农业领域青年科技创新人才培养资助计划(2014056) （2014056）
