中国油料作物学报2017，Vol.39Issue(3)：393-398,419,7.DOI:10.7505/j.issn.1007-9084.2017.03.015
菜豆壳球孢侵染芝麻过程中内参基因的筛选
Screening of reference genes in sesame during Macrophomina phaseolina infection
摘要
Abstract
To find out the accurate reference genes for gene expression analysis of Macrophomina phaseolina by qRT-PCR,11 housekeeping genes were detected in the pathogenic process.According to PCR amplification efficiencies,8 housekeeping genes were retained and their expression stabilities were evaluated by computer algorithms GeNorm.The 8 genes included ubiquitin-conjugating enzyme gene UBC,glyceraldehyde-3-phosphate dehydrogenase gene GAPDH,ribosomal protein S5 gene RPS5 (as RPS5-a and RPS5-b),α-tubulin gene TUBA,β-actin gene ACTB,γ-tubulin gene TUBC and internal transcribed spacer ITS.Using qRT-PCR,the expression stability of the 8 genes from sesame samples were investigated at 8 h,16 h,24 h and 32 h post M.phaseolina inoculation.GeNorm analysis revealed that the most stable genes were ACTB,GAPDH and RPS5-b.They showed their housekeeping genes features,and could be used jointly as reference genes of M.phaseolina for plant-microb interaction analysis.关键词
芝麻茎点枯病菌/qRT-PCR/内参基因/芝麻Key words
Macrophomina phaseolina (M.phaseolina)/qRT-PCR/reference gene/sesame分类
农业科技引用本文复制引用
赵辉,张春艳,文艺,刘玉霞,刘新涛,倪云霞,王飞,刘红彦..菜豆壳球孢侵染芝麻过程中内参基因的筛选[J].中国油料作物学报,2017,39(3):393-398,419,7.基金项目
国家自然科学基金(31301631) （31301631）
农业部现代农业产业技术体系(CARS-15-1-05) （CARS-15-1-05）
