作物学报2017，Vol.43Issue(8)：1115-1121,7.DOI:10.3724/SP.J.1006.2017.01115
寄主诱导的基因沉默提高植物真菌病害抗性研究进展
Progress in Enhancement of Plant Resistance against Fungal Diseases through Host-Induced Gene Silencing
摘要
Abstract
Host-induced gene silencing (HIGS) uses the key genes that are involved in growth and development, sporulation and propagation, and pathogenesis of pathogens as targets, and expresses the RNAi constructs in host plants targeting to the target genes; during infection of plants, pathogens take up dsRNA or siRNA molecules, specifically bind to nucleotide sequences after recognition, and interfere the expression of the target genes, thereby inhibiting infection and spreading of invaded pathogens and thus conferring resistance phenotypes in plants. This technology serves as the solid foundation of plant resistance based on patho-gen-specific sequences and has great potential for application in crop improvement against fungal diseases. In this review, recent advances in methodologies, technological routes and application of the HIGS strategy in plants against invading fungal pathogens are summarized; prospects for the future in HIGS-based plant resistance to fungal pathogens were also discussed.关键词
RNA干扰/寄主诱导的基因沉默(HIGS)/真菌病害/转基因植物Key words
RNA interfering/Host-induced gene silencing (HIGS)/Fungal disease/Transgenic plants引用本文复制引用
程伟,李和平,何水林,廖玉才..寄主诱导的基因沉默提高植物真菌病害抗性研究进展[J].作物学报,2017,43(8):1115-1121,7.基金项目
本研究由国家转基因生物新品种培育重大专项(2016ZX08002001-003), 国家自然科学基金项目(31601761)和福建省中青年教师教育科研项目(JAT160180)资助.This research was supported by the National Major Project for Developing New GM Crops (2016ZX08002001-003), the National Natural Science Foundation of China (31601761), and the Research Project for Young Teachers of Fujian Province (JAT160180). （2016ZX08002001-003）
