作物学报2017，Vol.43Issue(8)：1122-1127,6.DOI:10.3724/SP.J.1006.2017.01122
水稻颖花持续开放突变体sostenuto floret opening(sfo1)的鉴定与基因定位
Characterization and Gene Mapping of sostenuto floret opening 1 (sfo1) Mutant in Rice (Oryza sativa L.)
摘要
Abstract
Rice floret opening is one of the most critical physiological processes in its reproductive development, which has a significant influence on the fertilization and subsequent seed development. Rice floret opening and closing are promoted respec-tively by the lodicules' expansion and shrink. In recent years, many studies focused on the molecular mechanism of rice lodicule development. However, few studies focused on the molecular regulation mechanism in physiological process of lodicule opening and closing. In this paper, we reported a floret opening mutant, sostenuto floret opening 1 (sfo1), derived from EMS (ethyl meth-ane sulfonate) mutation groups of Xinong 1B. During the floret opening stage, the florets of sfo1 and wild type were observed directly by both the stereoscope and the scanning electron microscopy at about 1 h before opening, and 10 min, 1.5 h and 48 h after opening respecting. Compared with that in the wild type, the sfo1 floret showed a delay of lodicules dehydration after floret opening, resulting in its failing to close the lemma and palea. Significant differences were not found at 1 h before opening, show-ing closed hulls, flat lodicules and well-stacked lodicules surface cells. At 10 min after opening, there were also no obvious dif-ferences between wild type and mutants, showing opening hulls, inflated lodicules, and smooth and well-stacked surface cells. At 1.5 h after opening, sfo1 hulls were not closed so big as those in the wild type. And sfo1 lodicules still inflated, but not dehydrated and atrophied as those in the wild type. At 48 h after opening, sfo1 hulls were not closed yet, its lodicules began to dehydrate and atrophy, but were still very full compared with those in the wild type. The F1 and F2 of a cross with sterile lines 56S as female parent and the sfo1 as male parent were used in genetic analysis and gene mapping, indicating that the sfo1 trait was controlled by a nuclear recessive gene. Using bulked segregation analysis (BSA) method, the SFO1 was located between the SSR marker RM1054 and Insert/Delete marker ZTQ51 on the chromosome 5, with a physical distance of 113 kb and including 15 candidate genes in this region. LOC_Os05g50890 and LOC_Os05g50900 were preliminary selected as candidate genes for SFO1. These results laid a foundation for the SFO1 gene cloning and functional research.关键词
水稻/颖花开放/浆片/基因定位Key words
Rice (oryza sativa)/floret opening/Lodicule/Gene mapping引用本文复制引用
沈亚林,庄慧,陈欢,曾晓琴,李香凝,张君,郑昊,凌英华,李云峰..水稻颖花持续开放突变体sostenuto floret opening(sfo1)的鉴定与基因定位[J].作物学报,2017,43(8):1122-1127,6.基金项目
本研究由国家自然科学基金项目(31271304)和中央高校基本科研业务费(XDJK2016A013)资助.This study was supported by the National Natural Science Foundation of China (31271304) and Fundamental Research Funds for the Central Universities (XDJK2016A013). （31271304）
