作物学报2017，Vol.43Issue(8)：1128-1138,11.DOI:10.3724/SP.J.1006.2017.01128
玉米SNAC基因的遗传变异及耐旱性调控
Genetic Variations and Drought Tolerance of SNAC Genes in Common Maize Inbred Lines of China
摘要
Abstract
The coding regions and their upstream 800 bp promoter regions of SNAC genes (Sress-responsive NAM, ATAF1/2, CUC2) were sequenced in 16 maize inbred lines commonly used in China. Among 12 SNAC genes, genetic variations in promoter region were only identified in four SNAC genes, and more than 30 variations were identified in four SNAC genes, showing higher polymorphism in the four genes than other in SNAC genes. Although most of the SNAC genes were mainly SNP (Single nucleo-tide polymorphism) mutations, more insertion/deletion mutations were detected in ZmNAC031467 gene, reaching 63.3% of the total genetic variations. The PLACE software was used to predict three kinds of stress-tolerant binding elements in SNAC gene, but little effect was found to be related with the variations. Additionally, high nucleotide polymorphisms were identified in seven SNAC genes, especially with the highest π value of 0.00962 in ZmNAC030308, which suggested that they were greatly influenced by natural selection in the genetic drift. With the t-test, two mutations of ZmNAC070395 and ZmNAC080398 genes were associated with drought-tolerant traits, which provides references for further analysing the relationship between nucleotide variation in SNAC and drought tolerance traits.关键词
玉米/SNAC基因/遗传变异/耐旱性Key words
Zea mays L./SNAC genes/Genetic variations/Drought tolerance引用本文复制引用
李国君,马艺文,徐丹阳,吴永波,宋洁,王楠,郝转芳,赵娟..玉米SNAC基因的遗传变异及耐旱性调控[J].作物学报,2017,43(8):1128-1138,11.基金项目
本研究由国家自然科学基金重大国际合作项目(31661143010)和面上项目(31271735)资助.This study was supported by the National Natural Science Foundation of China (31661143010, 31271735). （31661143010）
