作物学报2017，Vol.43Issue(8)：1139-1148,10.DOI:10.3724/SP.J.1006.2017.01139
玉米InDel标记20重PCR检测体系的建立
Establishment of Multiplex PCR System with 20 Pairs of InDel Markers in Maize
摘要
Abstract
In order to improve the detection efficiency with molecular marker, the multiple PCR detection system was constructed. In this study, 10 major materials were used to evaluate the single pair PCR with 238 pairs of InDel primers. According to the software quality evaluation results, product range, and the principle of chromosome uniform distribution, 30 pairs of primers were selected from 192 primers with better performance to form two groups of core primer combinations with amplified products in the range of 80-200 bp and 200-400 bp, There were 10 pairs of primers distributing in different chromosomes for each primer com-bination. Based on core primer combination and comprehensive consideration on chromosome distribution, base fragment, and primers fluorescent color, we established two groups of corn test 20 PCR system and a group of 40 fluorescent capillary electro-phoresis.关键词
玉米/InDel/多重PCRKey words
Maize/InDel/Multiplex PCR引用本文复制引用
冯博,金石桥,张力科,蔚荣海,赵久然,许理文,王凤格,薛宁宁,刘文彬,易红梅,田红丽,吕远大,赵涵..玉米InDel标记20重PCR检测体系的建立[J].作物学报,2017,43(8):1139-1148,10.基金项目
本研究由国家科技支撑计划项目(2015BAD02B02)和北京市农林科学院科技创新能力建设专项(KJCX20161501)资助.This study was supported by the National Science and Technology Project from the Ministry of Science and Technology of China (2015BAD02B02), and the Science and Technology Innovation Special Project of Beijing Academy of Agriculture and Forestry Sciences (KJCX20161501). （2015BAD02B02）
