作物学报2017，Vol.43Issue(8)：1161-1169,9.DOI:10.3724/SP.J.1006.2017.01161
大豆转录因子基因GmNF-YCa可提高转基因拟南芥渗透胁迫的耐性
Soybean Transcription Factor Gene GmNF-YCa Enhances Osmotic Stress Tole-rance of Transgenic Arabidopsis
摘要
Abstract
Biotic stresses, like plant diseases and insect pests, and abiotic stresses, such as drought and salt, heavily threaten plant growth and influence crop yield and quality. Osmotic stress resulting from water deficiency in the soil is one of major hurdles. Nuclear Factor Y (NF-Y) is a heterotrimeric protein, consisting of NF-YA, NF-YB, and NF-YC in plant. NF-Y plays significant roles in the pathway responding to osmotic stresses in plants. We acquired a gene GmNF-YCa, a member of regulatory subunit NF-YC family in soybean (Glycine max L.), by screening a soybean cDNA library using yeast two-hybrid system. The full se-quence of GmNF-YCa is 864 bp, encoding 287 amino acids and having a NF-YC motif, belonging to NF-YC subfamily. GmNF-YCa is a hydrophilic protein with a molecular weight of 31.6 kD and isoelectric point of 5.07, and containing a transmem-brane domain and no any signal peptides. Sequence analysis showed that NF-YC subfamily was highly conserved among various species. Promoters of GmNF-YCa contained various abiotic stresses and light responsive elements, such as ARE, Box 4, GATA-motif, Box I, ACE, ABRE, and CAT-Box. According to tissue-specific analysis, GmNF-YCa had the highest expression level in germination stage. Quantitative real-time PCR suggested that GmNF-YCa was induced by sucrose stress and mannitol treatment. GmNF-YCa was transformated to Arabidopsis successfully by Agrobacterium-mediated method and the overexpressed Arabidopsis was prepared for the function characterization analysis. Overexpression of GmNF-YCa could improve tolerance of transgenic Arabidopsis to osmotic stress in the germination stage, and enhance root development with more lateral roots in su-crose and mannitol treatments.关键词
大豆/核因子Y/表达模式/启动子/渗透胁迫Key words
Soybean/Nuclear Factor Y/Expression pattern/Promoter/Osmotic stress引用本文复制引用
李敏,于太飞,徐兆师,张双喜,闵东红,陈明,马有志,柴守诚,郑炜君..大豆转录因子基因GmNF-YCa可提高转基因拟南芥渗透胁迫的耐性[J].作物学报,2017,43(8):1161-1169,9.基金项目
本研究由国家转基因生物新品种培育重大专项(2014ZX0800916B)和国家自然科学基金项目(31371620)资助.This study was supported by the National Major Project for Developing New GM Crops (2014ZX0800916B) and the National Natural Science Foundation of China (31371620). （2014ZX0800916B）
