作物学报2017，Vol.43Issue(8)：1170-1180,11.DOI:10.3724/SP.J.1006.2017.01170
红麻EST-SSR标记的开发及其多态性评价
Development and Polymorphism Evaluation of EST-SSR Markers in Kenaf
摘要
Abstract
Kenaf is one of the most important crops for natural fiber production. However, the lack of SSR markers has resulted in a large gap in kenaf genetic improvement. In this study, 94 SSR primers were developed on the basis of the sequences containing transcription factors from 90175 expressed sequence tags (ESTs) in kenaf. A total of 24 kenaf accessions were used to evaluate the polymorphism of these developed SSRs using 9% polyacrylamide gel electrophoresis. Eighty-five SSR primers (90.4%) were successfully amplified and presented the polymorphism between at least two accessions, indicating their high rate of polymor-phism. Among them, AAT and ATG were the dominant repeat motifs. Cluster analysis showed that the genetic similarity coeffi-cient varied from 0.62 to 0.92, indicating an abundant genetic basis among these accessions. These developed EST-SSRs not only enrich molecular markers, but also enhance the resource for genetic analysis in kenaf.关键词
红麻/SSR/遗传多样性/标记开发Key words
Kenaf (Hibiscus cannabinus)/Simple sequence repeat/Genetic diversity/Marker development引用本文复制引用
万雪贝,李东旭,徐益,徐建堂,张力岚,张列梅,林荔辉,祁建民,张立武..红麻EST-SSR标记的开发及其多态性评价[J].作物学报,2017,43(8):1170-1180,11.基金项目
本研究由福建农林大学杰出青年科研基金项目(xjq201401), 国家现代农业产业技术体系建设专项(CARS-19-E06), 农业部东南黄红麻实验观测站(农科教发 2011), 福建省科技厅对外合作项目(2015I0001)和福建省麻类种质资源共享平台项目(2010N2002)资助.This study was supported by the Distinguished Young Research Fund in Fujian Agriculture and Forestry University (xjq201401), the China Agricul-ture Research System (CARS-19-E06), the Experiment Station of Jute and Kenaf in Southeast China (Nongkejiaofa 2011), Foreign Cooperation Pro-ject of Science and Technology Department in Fujian, China (2015I0001), and Construction of Germplasm Resources Platform for Bast Fiber Crops in Fujian, China (2010N2002). （xjq201401）
