作物学报2017，Vol.43Issue(8)：1181-1189,9.DOI:10.3724/SP.J.1006.2017.01181
木薯转录因子基因MeHDZ14的克隆与分析
Cloning and Analysis of the Transcription Factor Gene MeHDZ14 in Cassava
摘要
Abstract
HD-Zip family genes play important roles in plant growth and stress response. To reveal the role of MeHDZ14 gene in abiotic stresses (e.g. drought) in cassava, we cloned MeHDZ14 gene by using RT-PCR from cassava cultivar SC124, which was relatively more resistant to drought stress. Bioinformatics methods were used to analyze its structural characteristics, and semi-quantitative RT-PCR/qRT-PCR was used to explore its expression patterns in response to abiotic stresses in different plant tissues and varieties. MeHDZ14 has a 726 bp open reading frame, encoding 241 amino acids, and contains the typical HD and ZIP domain. Blastp analysis showed that MeHDZ14 has close genetic relationship with ATHB-7, which is a member of the family I HD-Zip gene. Yeast and subcellular localization test showed that the MeHDZ14 gene is a transcription factor and specifically ex-presses in the nucleus. Genetic structural variation analysis revealed a total of four mis-sense mutations in eight tested varieties. However, amino acid mutations were not found between wild and cultivated cassavas. This indicates the MeHDZ14 proteins are highly conserved. Semi-quantitative RT PCR analysis revealed that MeHDZ14 was specifically expressed in petioles, and induced by drought stress in root and leaf, suggesting that MeHDZ14 plays an important role in the early drought stage. Analysis by qRT-PCR showed that MeHDZ14 gene had different expression levels in different cassava varieties, but the same mode under drought stress and ABA treatment. These data indicate that MeHDZ14 is a member of the ABA pathway responding to drought. Our results showed that MeHDZ14 plays an important role in the molecular pathways of cassava drought resistance, underlining its potential in genetic improvement of cassava drought tolerance.关键词
木薯/MeHDZ14/结构特性/表达分析Key words
Cassava/MeHDZ14/Structure characteristics/Expression analysis引用本文复制引用
于晓玲,阮孟斌,王斌,杨义伶,王树昌,彭明..木薯转录因子基因MeHDZ14的克隆与分析[J].作物学报,2017,43(8):1181-1189,9.基金项目
本研究由国际科技合作与交流项目(31561143012, 2013DFA32020)和国家自然科学基金项目(31501378)资助.This study was supported by the International Science & Technology Cooperation Program of China (31561143012, 2013DFA32020) and the National Natural Science Foundation of China (31501378). （31561143012, 2013DFA32020）
