中国中西医结合杂志2017，Vol.37Issue(7)：775-780,6.DOI:10.7661/j.cjim.20170426.091
基于miR-155/NF-κB信号通路探讨类风湿关节炎患者血瘀状态与肺功能的关系
Relationship between Blood Stasis State and Pulmonary Function in Patients with Rheumatoid Arthritis Based on miR-155/NF-κB Signaling Pathway
摘要
Abstract
To study the relationship between blood stasis state and pulmonary function in patients with rheumatoid arthritis (RA) based on miR-155/NF-kappa B signaling pathway.Methods Sixty RA patients were recruited,and 20 subjects were recruited in a healthy control group.German Jager MasterScreen automatic lung function tester was used to detect of pulmonary function parameters.And the levels of IL-10,IL-6,IL-4,IL-17,NF-κB activator 1 (Act1),p50,p65,NF-κB inhibitory protein α(IκBα),platelet activator factor (PAF),PAF-AH were detected by ELISA.In addition,platelet (PLT) was analyzed via automatic blood cell analyzer.And the levels of D-dimer (D-D) and fibrinogen (FBG) were tested by automatic coagulation test.Furthermore,the expression levels of protein Act1,p65,p50,and IκBα were also tested by Western blot.The miR-155 of peripheral blood was detected by one step fluorescent Q-PCR.Spearman analysis was used to analyze the correlation between blood stasis indices,cytokines,NF-κB,miR-155 and pulmonary function parameters in peripheral blood of RA patients.Results Compared with NC group,the levels of IL-6,IL-17,miR-155,and PAF in RA group were significantly increased,protein expressions of Act1,p50,p65 and IκBα were significantly increased,but the levels of PAF-AH,IL-10,and IL-4 were significantly decreased (P ＜0.05,P ＜0.01).Compared with mild blood stasis syndrome group,FEV1/FVC,FEF50,FEF75 were significantly decreased,protein expression of p65 and miR-155 were significantly increased in severe blood stasis syndrome group.And Spearman analysis showed that there was a correlation between blood stasis indices,cytokines,NF-κ B,miR-155,and pulmonary function parameters.Conclusion Blood stasis state in RA patients is closely related to abnormal pulmonary function,which may be related to high expression of miR-155 in vivo,abnormal activation of NF-κB,and the imbalance of cytokine network.关键词
类风湿关节炎/血瘀状态/核因子-κB/肺功能Key words
rheumatoid arthritis/blood stasis state/nuclear factor-κB/pulmonary function引用本文复制引用
章平衡,刘健,黄旦,万磊..基于miR-155/NF-κB信号通路探讨类风湿关节炎患者血瘀状态与肺功能的关系[J].中国中西医结合杂志,2017,37(7):775-780,6.基金项目
国家自然科学基金资助项目(No.81173211/H2708) （No.81173211/H2708）
国家自然科学基金资助项目(No.81403388) （No.81403388）
安徽省自然科学基金资助项目(No.1508085QH159) （No.1508085QH159）
安徽省重点实验室:现代中医内科应用基础与开发研究项目(No.1606c08238) （No.1606c08238）
安徽中医学院科技创新团队项目(No.2010TD005) （No.2010TD005）
安徽中医药大学校级基金项目(No.2014qn025) （No.2014qn025）
