中国中西医结合杂志2017，Vol.37Issue(7)：781-784,4.DOI:10.7661/j.cjim.20170515.092
青风藤汤联合甲氨蝶呤对类风湿关节炎患者滤泡辅助性T细胞及白介素-21的影响
Effect of Qingfengteng Decoction Combined with Methotrexate on Peripheral Blood Follicular Helper T Cell and Interleukin-21 in Rheumatoid Arthritis Patients
摘要
Abstract
To observe the effect of Qingfengteng Decoction (QFTD) combined with Methotrexate (MTX) on peripheral blood follicular helper T cell (Tfh),IL-21,and disease activity in rheumatoid arthritis (RA) patients at the first visit.Methods Totally 72 early RA patients were randomly assigned to the research group and the control group,36 cases in each group.All patients took MTX (10 mg once a week) and Celecoxib (0.2 g/capsule,twice per day;the dosage of celecoxib was tapered according to clinical symptoms).Patients in the research group additionally took QFTD.The treatment course for all was 12 weeks.Erythrocyte sedimentation rate (ESR) and Disease Activity Score in 28 joints (DAS28)were measured before treatment,at week 4,8,and 12 weeks.Tfh count in peripheral blood was detected by flow cytometry before treatment and at week 12 after treatment.Serum contents of IL-21 and anti-cyclic citrullinated peptide (anti-CCP) antibody were detected by enzyme linked immunosorbent assay (ELISA) before and after treatment.Results Compared with before treatment in the same group,ESR and DAS28 in the both two groups decreased at week 4,8,and 12 (P ＜0.01).DAS28 of the research group was lower than that in the control group at week 12 (P ＜0.01).There was no significant difference in anti-CCP antibody in the two groups between before treatment and at week 12 after treatment (P ＞0.05).Compared with the healthy population,the proportion of Tfh/CD4+T and the IL-21 level increased in the two groups before treatment (P ＜0.01).Compared with before treatment in the same group,the proportion of Tfh/CD4+T and the IL-21 level decreased in the two groups at week 12.Besides,they were lower in the research group than in the control group (P ＜0.01,P ＜0.05).Conclusion QFTD combined with MTX could decrease Tfh and IL-21 level in RA patients,and improve clinical symptoms of KOA patients.关键词
类风湿关节炎/青风藤汤/滤泡辅助性T细胞/白介素-21Key words
rheumatoid arthritis/Qingfengteng Decoction/follicular helper T cell/interleukin-21引用本文复制引用
许超,张芳,吴倩,杨宁,杨文彦,耿洁,钱先..青风藤汤联合甲氨蝶呤对类风湿关节炎患者滤泡辅助性T细胞及白介素-21的影响[J].中国中西医结合杂志,2017,37(7):781-784,4.基金项目
中国中医科学院江苏分院开放课题项目(No.FY201509) （No.FY201509）
