中国中西医结合杂志2017，Vol.37Issue(7)：858-862,5.DOI:10.7661/j.cjim.20170428.094
密蒙花颗粒剂对去势雄兔泪腺细胞凋亡因子Bax、Bcl-2、Fas和FasL的影响
Effect of Buddleia Granule on Bax, Bcl-2, Fas, and FasL of Lacrimal Gland Cells of Castrated Male Rabbits
摘要
Abstract
To observe the effect of Buddleia Granule (BG) on apoptosis factors (Bax,Bcl-2,Fas,and FasL) of lacrimal gland cells of castrated male rabbits,and to study its effect on dry eye model of castrated male rabbits.Methods Buddleia raw materials were made into granules.Totally 30 healthy adult New Zealand rabbits were randomly divided into blank group (Group A),model group (Group B),BG group (Group C),the placebo group (Group D),testosterone group (Group E),6 rabbits in each group.Bilateral testis and epididymis were cut off to rabbits of Group B to E.The rabbits of Group C were given BG according to 100 mg/kg by gastrogavage,3 times per day.Those in Group D were given normal saline,20 mL each time,3 times per day.Those in Group E were injected with Testosterone Propionate Injection (2 mg/kg) in thigh muscle,once injection per every 3 days.All rabbits were tested Schirmer Ⅰ test (S I T) and tear break-up time (BUT) before operation and 4 weeks after operation.After 4 weeks,all rabbits were scarified by air embolism and bilateral lacrimal glands clipped.Expressions of inflammatory factors (Bax,Bcl-2,Fas,and FasL) from lacrimal gland cells were checked by immunohistochemical staining method.The morphology of lacrimal gland was also observed.Results (1) Resuits of S I T and BUT:Compared with before operation and Group A after operation,S I T and BUT were significantly decreased in Group B and D after operation (P ＜0.01).After operation,as compared with Group B and D,S Ⅰ T and BUT were significantly increased in Group C and E,with statistical difference (P ＜0.01).(2) Expressions of Bax,Bcl-2,Fas,and FasL:Compared with Group A,the expression of Bax,Fas,and FasL were obviously increased,but Bcl-2 was decreased obviously in Group B and D (P ＜0.01).Compared with Group B and D,the expressions of Bax,Fas,and FasL were decreased,but Bcl2 was increased obviously in Group C and E,with statistical difference (P ＜0.01).(3) The results of Bax,Bcl-2,Fas,FasL expressions:Group A:The structure of lacrimal gland was clear.The expressions of Bax,Bcl-2,Fas,FasL were not found.Group B and D:The structure of lacrimal gland was indistinct.Bax,Fas,and FasL were expressed in cell membrane and cytoplasm heavily,showing brownish granules with yellow.The expression of Bcl-2 was only expressed in a large amount.Group C:The structure of lacrimal gland was clear.The expression of Bax,Bcl-2,Fas,and FasL were expressed scarcely.Bcl2 was expressed in cell membrane and cytoplasm heavily,showing brownish granules with yellow.Group E:The structure of lacrimal gland was clear.The expression of Bax,Bcl-2,Fas,and FasL were expressed in a small amount.Bcl-2 was not found.Conclusions Compared with androgen,BG had a similar effect of depressing the expression of Bax,Fas,FasL and increasing the expressions of Bcl-2.lt could inhibit the apoptosis of lacrimal gland cells in castrated male rabbits,maintain the basic secretion.But its effect was slightly weaker than that of the androgen.关键词
密蒙花颗粒剂/去势雄兔/泪腺细胞/细胞凋亡因子Key words
Buddleia Granule/castrated male rabbit/lacrimal gland cell/apoptosis factor引用本文复制引用
欧阳云,彭俊,彭清华,谭涵宇,李文娟,吴权龙..密蒙花颗粒剂对去势雄兔泪腺细胞凋亡因子Bax、Bcl-2、Fas和FasL的影响[J].中国中西医结合杂志,2017,37(7):858-862,5.基金项目
国家自然科学基金面上资助项目(No.30772824,No.81574031) （No.30772824,No.81574031）
湖南省高层次卫生人才“225”工程培养项目资助 （）
湖南省科技厅科研基金资助项目(No.2015SF2016-6) （No.2015SF2016-6）
湖南省发展改革委科研基金资助项目(湘发改投资[2014]658号) （湘发改投资[2014]658号）
长沙市科技计划重大专项(No.K1501014-31) （No.K1501014-31）
