安徽农业科学2017，Vol.45Issue(21)：4-6,187,4.
一株来源于土壤的耐盐酵母菌鉴定及其与植物互作研究
Identification of A Strain of Salt-tolerance Halophilic Yeast from Soil and Its Interaction with Plant
摘要
Abstract
[Objective] The aim was to identify a strain of salt-tolerance halophilic yeast from soil and study its interaction with plant.[Method] A halophilic yeast YSTEn005 was isolated from the Pinus massoniana rhizosphere soil in Enshi,and it interaction with plant was explored under salt stress.[Result] YSTEn005 could grow on the medium supplemented with up to 10% NaCl,and it appeared in sensitive to K+;the optimum growth temperature of YSTEn005 was 28 ℃,and growth rate was higher in medium with 4% NaCl than other series of degrees;YSTEn005 was tolerant to wide pH relatively,which ranged from 4.0 to 9.0.YSTEn005 was single cell pattern under microscope,and displayed budding similar to that of yeast.Cloning its 18S rDNA and analysis the sequence indicated that the YSTEn005 was evolutionally close to Debaryomyces hansenii,which is a halophilic yeast,suggesting that YSTEn005 was from this genus.When co-cultured with plants,YSTEn005 growth promoted Arabidopsis seedlings dying significantly under salt stress conditions.[Conclusion] A strain of halophilic yeast YSTEn005 is obtained from soil,and the YSTEn005 growth is not helpful to plants tolerant to salt stress.关键词
真菌/耐盐酵母菌/汉逊德巴利酵母/土壤Key words
Fungi/Halophilic yeast/Debaryomyces hansenii/Soil分类
农业科技引用本文复制引用
毛慧芳,彭杰娣,杨棨博,周毅峰,田国政,刘晓鹏,唐巧玉,方庆..一株来源于土壤的耐盐酵母菌鉴定及其与植物互作研究[J].安徽农业科学,2017,45(21):4-6,187,4.基金项目
湖北民族学院博士启动基金项目:(4148013) （4148013）
国家自然科学基金项目:(31360498). （31360498）
