北京林业大学学报2017，Vol.39Issue(7)：31-39,9.DOI:10.13332/j.1000--1522.20170055
外源BR与IAA对欧美杨耐旱性的影响
Effects of exogenous BR and IAA on drought tolerance of Populus deltoides × P. nigra
摘要
Abstract
In order to study the effects of exogenous BR and IAA on the drought resistance of poplar, the 1-year-old potted seedlings of three Populus deltoides × P. nigra clones, NE19, R270, and 107 were selected for 15 days of treatment and sprayed with 10μmol/L brassinostevoid ( BR) and 100 mg/L IAA. The results showed that BR and IAA significantly improved the net photosynthetic rate ( Pn ) , photochemical quantum yield ( Fv/Fm ) , chlorophyll content, leaf water-holding capacity under drought condition compared with the hormone-free. The Pn of NE19, R270 and 107 was increased by 19%, 60%and 85%, respectively in BR treatment, and 35%, 50% and 80%, respectively in IAA treatment. The Fv/Fm of BR group increased by 7. 7%, 7. 5% and 10. 9% compared with the non-hormone group, while the IAA group increased by 7. 4%, 8% and 11. 9%, respectively. The results indicated that exogenous BR and IAA improved the drought tolerance of Populus deltoides × P. nigra clones. Examining the leaf discs treated with exogenous hormones treatment and it was indicated that, for all three clones, the stomatal indix decreased by 12. 28%, 25. 60% and 20. 31%, respectively in BR treatment, and the IAA treatment decreased by 13. 00%, 13. 15% and 14. 48%, respectively. BR and IAA treatments inhibit stomatal development to reduce water loss under drought conditions. The expressions of EPF1, EPF2, EPFL9, ERECTA, FAMA, SDD1 in stomatal growth-related genes were detected by fluorescence quantitative PCR. EPF1, EPF2, ERECTA and SDD1 which negatively control stomatal development were increased, but the expressions of FAMA and EPFL9 which positively regulate the process were decreased. In summary, phytohormone BR and IAA are involved in the process of stomatal development in Populus deltoides × P. nigra, and increase drought resistance of poplar by inhibiting stomatal development under drought conditions.关键词
BR/IAA/欧美杨/外源激素/耐旱性Key words
BR/IAA/Populus deltoides × P. nigra/exogenous hormone/drought tolerance分类
农业科技引用本文复制引用
贾伏丽,王丛鹏,刘沙,焦志银,尹伟伦,夏新莉..外源BR与IAA对欧美杨耐旱性的影响[J].北京林业大学学报,2017,39(7):31-39,9.基金项目
"十二五"国家科技支撑计划项目(2015BAD07B01)、国家自然科学基金项目(31270656). （2015BAD07B01）
