大豆科学2017，Vol.36Issue(4)：547-553,7.DOI:10.11861/j.issn.1000-9841.2017.04.0547
生长素、乙烯和温度对大豆不定根形成的影响
Coordinated Effects of IAA and Ethylene and Temperature on Adventitious Root Organogenesis in Soybean Hypocotyl Cuttings
摘要
Abstract
In present study,hypocotyl cuttings from soybean variety DN594 with few branch and CHARLESTON with a few branches were observed in the solutions of different concentrations of IAA,ethylene (ethylene releasing agent),mixture of both IAA and ethephon.Optimal concentrations of different solutions were determined,and then fluorescence quantitative PCR was used to measure the genic expression of genes related to IAA and ethane with high and low temperature.The results could provide more genetic evidences about gene expression of exogenous hormones during the formation of soybean adventitious root under different temperature.The results were as follows:Low concentration of phytohormones promoted the formation of soybean adventitious root,while high concentration inhibited the process.Different phenotypes were observed between vary soybean genotypes,the phenotypes treated with mixture of IAA and ethylene were different with that of single hormone.Gene expression also varied between two genotypes and could be influenced by temperature.Genes related to ethylene could regulate the expression of genes associated to IAA.关键词
大豆/不定根/IAA/乙烯利/温度Key words
Soybean/Adventitious root/IAA/Ethylene/Temperature分类
农业科技引用本文复制引用
刘凤丽,王万鹏,李文滨,刘珊珊,姜振峰..生长素、乙烯和温度对大豆不定根形成的影响[J].大豆科学,2017,36(4):547-553,7.基金项目
国家自然科学基金(31571693) （31571693）
国家现代农业产业技术体系(CARS-04-PS04) （CARS-04-PS04）
黑龙江省博士后科研启动金项目(LBH-Q14021) （LBH-Q14021）
黑龙江省教育厅科学研究项目(12521008). （12521008）
