动物营养学报2017，Vol.29Issue(8)：2707-2715,9.DOI:10.3969/j.issn.1006-267x.2017.08.013
体外培养条件下不同长链脂肪酸对山羊瘤胃原虫群体结构和原虫吞噬细菌循环的影响
Effects of Different Long-Chain Fatty Acids on Protozoal Community Structure and Bacterial Recycling Due to Protozoa Engulfment of Goat Rumen in Vitro
摘要
Abstract
This experiment was conducted to investigate the effects of different long-chain fatty acids on proto-zoal community structure and bacterial recycling due to protozoa engulfment of goat rumen in vitro. Six long-chain fatty acids with different saturations were selected, they were stearic acid ( group A) , oleic acid ( group B) , linoleic acid ( group C) , α-linoleic acid ( group D) , arachidonic acid ( group E) and eicosapentaenoic acid ( group F) , respectively, and the number of unsaturated bond of them was orderly increased from 0 to 5. The fatty acids above were used at a level of 3% in the substrate to perform the in vitro culture. The protozoal engulfing bacteria rate was assayed at 10 h of the in vitro cultured and the densities of protozoal and bacterial and the contents of the protozoal protein and bacterial protein were measured at 24 h of the in vitro culture. The results showed that, except the percentage of Ophryoscolecinae in group F was significantly higher than that in groups B and D ( P<0.05) , adding different long-chain fatty acids had no significant effects on the percentages of Dasytricharuminantium, Entodinium, Diplodiniinae, Isotrichidae and Epidinium ( P>0.05) . The protozoal density was the highest in group A which was significantly higher than that in group B ( P<0.05); while the bacterial density in group D was the highest and significantly higher than at in groups A, B, E and F ( P<0.05). The bacteria engulfing rate of protozoa in 5 groups was 244. 50 ( group A), 236. 51 ( group B), 229.60 ( group C) , 189.04 ( group D) , 200.51 ( group E) , 174.24 cells/( cell·h) ( group F) , respective-ly. Additionally, the bacteria recycling rate was the lowest in group D with the longest bacterial cell recycling time ( 146. 92 h) . It was further calculated that, the bacterial protein recycling amounts were comparatively lower in groups D [126.75 mg/(d·head)] and F [131.63 mg/(d·head)]. Base on this experiment, it is therefore considered that α-linoleic acid has better inhibitory effect on the bacterial recycling due to protozoa engulfment of goat rumen in vitro.关键词
脂肪酸/不饱和键/原虫/吞噬细菌Key words
fatty acids/unsaturated bond/protozoa/engulfing bacteria分类
农业科技引用本文复制引用
经语佳,高健,王梦芝,欧阳佳良..体外培养条件下不同长链脂肪酸对山羊瘤胃原虫群体结构和原虫吞噬细菌循环的影响[J].动物营养学报,2017,29(8):2707-2715,9.基金项目
国家自然基金项目(31672446) （31672446）
江苏省自然科学基金基础研究项目(BK20151312) （BK20151312）
江苏省优势学科 （）
