动物营养学报2017，Vol.29Issue(8)：2751-2761,11.DOI:10.3969/j.issn.1006-267x.2017.08.018
亚麻籽和鱼油对蛋鸡蛋黄n-3多不饱和脂肪酸 含量与肝脏脂肪酸代谢的影响
Effects of Dietary Flaxseed and Fish Oil on n-3 Polyunsaturated Fatty Acid Content in Egg Yolk and Fatty Acid Metabolism in Liver of Hens
摘要
Abstract
This experiment was conducted to investigate the effects of dietary flaxseed and fish oil on n-3 poly-unsaturated fatty acid content in egg yolk, fatty acid composition and fatty acid biosynthesis gene expression in liver of hens. Ninety-six Hyline Brown hens were divided into 4 groups with 24 hens per group. Hens in the control group were fed a basal diet, and those in trial groups were fed the basal diet+10% flaxseed, the basal diet+10% flaxseed+5% fish oil, and the basal diet+5% fish oil, respectively. Eggs were collected for 7 days after 21 days feeding, and then 4 hens in each group were slaughtered for samples. The results showed as fol-lows, compared with control group, 1) dietary flaxseed and fish oil significantly increased the n-3 polyunsatu-rated fatty acid contents in yolk ( P<0.05) , and contents of docosahexaenoic acid ( DHA) and timnodonic acid were the highest in yolk affected by dietary fish oil. 2) The liver monounsaturated fatty acid ratios were signifi-cantly decreased ( P<0.05) , and the liver n-3 polyunsaturated fatty acid ratios were significantly increased ( P<0.05) in all trial groups. 3) Dietary fish oil significantly decreased gene expressive levels of ELOVL fatty acid elongase 1, ELOVL fatty acid elongase 2 and fatty acid desaturase such as fatty acid desaturase 1, fatty acid desaturase 2 and stearoyl CoA desaturase 1 ( P<0.05) . In conclusion, dietary flaxseed or fish oil can concen-trate n-3 polyunsaturated fatty acid content in yolk of hens. Dietary flaxseed can promote generation process from α-linolenic acid to DHA, exhibiting up-regulating expressive levels of fatty acid elongase and desaturase genes. Dietary fish oil presented contrary results to flaxseed.关键词
亚麻籽/鱼油/n-3不饱和脂肪酸/DHA/蛋黄Key words
flaxseed/fish oil/n-3 polyunsaturated fatty acids/DHA/egg yolk分类
农业科技引用本文复制引用
邓波,门小明,朱冬荣,陶新,姚云昕,徐子伟..亚麻籽和鱼油对蛋鸡蛋黄n-3多不饱和脂肪酸 含量与肝脏脂肪酸代谢的影响[J].动物营养学报,2017,29(8):2751-2761,11.基金项目
浙江省重点科技创新团队项目(2011R50025-1) （2011R50025-1）
