动物营养学报2017，Vol.29Issue(8)：2762-2768,7.DOI:10.3969/j.issn.1006-267x.2017.08.019
枯草芽孢杆菌与锰协同对5~11周龄五龙鹅 血清生化指标、抗氧化能力及 胫骨发育的影响
Effects of Bacillus subtilis Cooperated with Manganese on Serum Biochemical Indices, Antioxidant Activity and Tibia Development of Wulong Geese Aged from 5 to 11 Weeks
摘要
Abstract
This experiment was conducted to study the effects of Bacillus subtilis cooperated with manganese on serum biochemical indices, antioxidant activity and tibia development of Wulong geese aged from 5 to 11 weeks, and to investigate the requirement of manganese when diet supplemented Bacillus subtilis of geese. A total of 360 five-week-old Wulong geese were randomly divided into 6 groups with 6 replicates per group and 10 geese per replicate ( half male and half female ) . Geese in group Ⅰ were fed a diet supplemented with 105 mg/kg manganese and without Bacillus subtilis; whereas the geese in groups Ⅱ to Ⅵ were fed diets all supplemented with 250 g/t Bacillus subtilis, and supplemented with 0, 35, 70, 105 and 170 mg/kg manga-nese, respectively. The experiment lasted for 7 weeks. The results showed as follows: 1) the serum alkaline phosphatase ( AKP) activity and the contents of triglyceride ( TG) and total cholesterol ( CHOL) in groupsⅡto Ⅵ were showed a firstly increased then decreased tendency with dietary manganese supplemental level in-creased, and no significant differences were found among all groups ( P>0.05) , the highest values were in the group Ⅳ. 2) The serum total antioxidant capacity ( T-AOC) and glutathione peroxidase ( GSH-Px) activity in groups Ⅱ to Ⅵ were showed a firstly increased then decreased tendency with dietary manganese supplemental level increased, the serum T-AOC and GSH-Px activity in group Ⅳ were significantly higher than those in group Ⅰ ( P<0.05) , and significantly higher than those in group Ⅱ ( P<0.01) . The serum malondialdehyde ( MDA) content in groupsⅡ to Ⅵ were showed a firstly decreased then increased tendency with dietary man-ganese supplemental level increased, the serum MDA content in group Ⅳ was significantly lower than that in group Ⅱ ( P<0.01) . 3) The bone mineral density, bone strength, tibia weight and tibia ash content in groupsⅡ toⅥwere showed a firstly increased then decreased tendency with dietary manganese supplemental level in-creased, the bone mineral density, bone strength, tibia weight and tibia calcium content in group Ⅳ were sig-nificantly higher than those in group Ⅰ ( P<0.05 or P<0.01) , the tibia manganese content in groupsⅡ, Ⅲ,Ⅳ and Ⅴ was significantly lower than that in group Ⅰ ( P<0.05 or P<0.01) . In conclusion, adding Bacillus subtilis into the diet can improve the biological effects of manganese, improve the body' s nutritional assimila-tion, promote antioxidant activity and tibia development, and reduce tibia manganese content. The optimal sup-plemental level of manganese was 70 mg/kg when dietary supplemented 250 g/t Bacillus subtilis of Wulong geese aged from 5 to 11 weeks.关键词
枯草芽孢杆菌/五龙鹅/锰/血清生化指标/胫骨发育/抗氧化能力Key words
Bacillus subtilis/Wulong geese/manganese/serum biochemical indices/tibia development/an-tioxidant activity分类
农业科技引用本文复制引用
葛文华,刁翠萍,任民,王宝维,张名爱,岳斌,郑惠文,张洋洋..枯草芽孢杆菌与锰协同对5~11周龄五龙鹅 血清生化指标、抗氧化能力及 胫骨发育的影响[J].动物营养学报,2017,29(8):2762-2768,7.基金项目
国家水禽产业技术体系专项基金(CARS-43-11) （CARS-43-11）
山东省良种工程(12-1-3-17-nsh) （12-1-3-17-nsh）
