动物营养学报2017，Vol.29Issue(8)：2817-2825,9.DOI:10.3969/j.issn.1006-267x.2017.08.026
大熊猫粪便中纤维素降解菌的筛选及 其产酶条件的优化
Screening of Celluloytic Bacterium from Giant Panda' s Feces and Optimization of Condition for Cellulase Production
摘要
Abstract
This experiment aimed to screen and identify the cellulose-decomposing bacterium isolated from the feces of giant pandas, and condition for producing cellulase was optimized. The cellulase-producing bacterium named DL was isolated from the feces of giant pandas, used the methods of sodium carboxymethylcellulose ( CMC-Na) as unique carbon source and iodine staining, disintegration of filter paper test and measurement of cellulase activity. Based on the morphological observation, physiological and biochemical characteristics and the result of sequences alignment of 16S rDNA, strain DL was identified as Paenibacillus cookii LZ033. The strain of Paenibacillus cookii LZ033 was found to be a spore-forming, gram-positive aerobic bacterium. Medi-um initial pH, culture temperature, shaker speed and liquid medium volume were chosen to determine the opti-mum condition for cellulase production. On the basis of the single factor experiment, the orthogonal experiment was used to analysis the optimum condition for cellulase production of strain DL, which was shown as follows:medium initial pH 6, culture temperature 35 ℃, rotation speed 125 r/min, broth liquid medium volume 100 mL in 250 mL flask. Under the optimum condition, the activity of cellulose ( expressed in filter paper ac-tivity) was up to 102.3 U/mL.关键词
大熊猫粪便/纤维素降解菌/产酶条件Key words
feces of giant pandas/celluloytic bacterium/conditions for cellulase production分类
生物科学引用本文复制引用
张智,尹文哲,雅男,马建章..大熊猫粪便中纤维素降解菌的筛选及 其产酶条件的优化[J].动物营养学报,2017,29(8):2817-2825,9.基金项目
国家林业局大熊猫国际资金项目"大熊猫益生菌制剂的研究"( SG1409) （ SG1409）
