动物营养学报2017，Vol.29Issue(8)：2961-2968,8.DOI:10.3969/j.issn.1006-267x.2017.08.041
丁酸梭菌对断奶仔猪生长性能、肠道屏障功能和 血清细胞因子含量的影响
Effects of Clostridium butyricum on Growth Performance, Intestinal Barrier Function and Serum Cytokine Contents of Weaned Piglets
摘要
Abstract
This study was aimed to investigate the effects of Clostridium butyricum on growth performance, in-testinal barrier function and serum cytokine contents of weaned piglets. A single factor design was used in this experiment, and 12 Duroc×Landrace×Yorkshire weaned piglets with similar body weight and good health were selected and divided into 2 groups at 28 days of age. Each group was divided into 6 replicates and each pig was fed in single column. Pigs in control group were fed a corn-soybean type basal diet, and those in experimental group were fed the basal diet supplemented with 5×105 CFU/g Clostridium butyricum for 14 days after 3 days adaption. The results showed as follows: compared with the control group, the average daily gain of piglets in experimental group was significantly increased by 7.83% (P<0.05), and the ratio of feed to gain was de-creased by 5.26% (P>0.05); the mRNA relative expression levels of NOD like receptor protein (NLRP) 3 (P<0.05), NLRP6 (P<0.05), NLRP12 (P<0.01), claudin-1 (P<0.01) and zonula occludens protein 2 ( ZO-2) ( P<0.05) were significantly or extremely significantly increased in jejunum and the mRNA relative expression levels of claudin-1 and ZO-2 were extremely significantly increased ( P<0.01) in ileum of piglets in experimental group; the number of Lactobacilli in the jejunum and ileum of piglets in experimental group was significantly increased ( P<0.05) , and the number of Escherichia coli in the jejunum of piglets in experimental group was decreased by 2.49% ( P>0.05) , while the number of Escherichia coli in the ileum of piglets in ex-perimental group was significantly decreased (P<0.05); the serum interleukin (IL)-1β content of piglets in experimental group was decreased by 5.47% ( P>0.05) , but the serum IL-10 content of piglets in experimental group was increased by 25.43% (P>0.05). In conclusion, dietary Clostridium butyricum can improve small intestinal barrier function of weaned piglets, regulate body immunity and the balance of intestinal flora, and thus promote weaned piglets growth.关键词
丁酸梭菌/断奶仔猪/生长性能/紧密连接/肠道屏障Key words
Clostridium butyricum/weaned piglets/growth performance/tight junction/intestinal mucosal barrier分类
农业科技引用本文复制引用
李玉鹏,李海花,王柳懿,朱琪,陈龙宾,乔家运,王文杰..丁酸梭菌对断奶仔猪生长性能、肠道屏障功能和 血清细胞因子含量的影响[J].动物营养学报,2017,29(8):2961-2968,8.基金项目
大北农杨胜先生门生社群项目( B2016008) （ B2016008）
