广东医学2017，Vol.38Issue(16)：2428-2431,4.
老年大鼠肾脏损伤中ASPP2蛋白调控与内质网应激的作用及相关性分析
The role of ASPP2 regulation and endoplasmic reticulum stress in renal injury in aged rats and their correlation.
摘要
Abstract
Objective To investigate the role of ASPP2 (one of P53 apoptosis stimulating protein family members) protein and endoplasmic reticulum stress in the kidney injury in aged rats.Methods The 6 months SD rats were assigned into adult group, and those of 18 months in elderly group.Hematoxylin eosin staining (HE) was used to observe the changes of renal structure.And mRNA expression levels of CHOP, ATF6, XBP-1 and GRP78 were detected by qRT-PCR method.Western blot was used to detect the protein expression level of ASPP2.The correlations between the protein expression of ASPP2 and endoplasmic reticulum stress related index CHOP, ATF6, XBP-1 and GRP78 mRNA expression levels were analyzed.Results Compared with adult group SD rats, kidney structure in elderly group SD rats was obviously changed, with significant reduction in CHOP, ATF6, XBP-1 and GRP78 mRNA expression and ASPP2 protein expression levels.The level of ASPP2 was positively correlated with CHOP and XBP-1, and negatively correlated with ATF6.Conclusion In elderly SD rats, the regulation of endoplasmic reticulum stress by ASPP2 may play important role in renal injury.关键词
P53凋亡刺激蛋白2/内质网应激/老年大鼠/肾脏Key words
ASPP2/endoplasmic reticulum stress/aged rats/kidney引用本文复制引用
杨松昊,田珏,杨晓玲,姜怡邓,张辉,杨安宁,侯苗苗,朱光荣,王楠,谢琳,丁宁,蒋袁絮..老年大鼠肾脏损伤中ASPP2蛋白调控与内质网应激的作用及相关性分析[J].广东医学,2017,38(16):2428-2431,4.基金项目
国家自然科学基金资助项目(编号:81360063,81460121) （编号:81360063,81460121）
