海南医学院学报2017，Vol.23Issue(12)：1593-1595,1599,4.DOI:10.13210/j.cnki.jhmu.20170627.016
杜仲补骨脂促进离体培养成骨细胞增殖的实验研究
Eucommia and fructus psoraleae promote the proliferation of osteoblasts cultured in vitro: an experimental study
摘要
Abstract
Objective: To study the effect of eucommia and fructus psoraleae treatment on the proliferation of osteoblasts cultured in vitro.Methods: One-day-old SD rats were taken, the osteoblasts in exposed bone were separated, cultured and divided into 4 groups, group A were treated with DMEM without drugs or serum, group B were treated with serum-free DMEM containing eucommia with final concentration 10 μg/L, group C were treated with serum-free DMEM containing fructus psoraleae with final concentration 10 μmol/L, and group D were treated with serum-free DMEM containing eucommia with final concentration 10 μg/L and fructus psoraleae with final concentration 10 μmol/L.Twenty-four hours after treatment, the mRNA expression of apoptosis genes and proliferation genes in cells were detected.Results:: Bax, Fas, FasL and HSG mRNA expression in group B, group C and group D were significantly lower than those in group A while c-fos, c-jun, CyclinD1, Egr-1 and NDRG1 mRNA expression were significantly higher than those in group A;Bax, Fas, FasL and HSG mRNA expression in group D were significantly lower than those in group B and group C, and c-fos, c-jun, CyclinD1, Egr-1 and NDRG1 mRNA expression were significantly higher than those in group B and group C.Conclusions: Both eucommia and fructus psoraleae can promote the osteoblast proliferation, and the combination of the two drugs has synergistic effect.关键词
骨质疏松/成骨细胞/杜仲/补骨脂/增殖Key words
Osteoporosis/Osteoblast/Eucommia/Fructus psoraleae/Proliferation分类
医药卫生引用本文复制引用
邹泽良,吴峰..杜仲补骨脂促进离体培养成骨细胞增殖的实验研究[J].海南医学院学报,2017,23(12):1593-1595,1599,4.基金项目
佛山市科技局项目(20150318)It was supported by the Project of Foshan Science and Technology Bureau (20150318). （20150318）
