海南医学院学报2017，Vol.23Issue(12)：1675-1678,4.DOI:10.13210/j.cnki.jhmu.20170627.012
miRNA-210在人脑胶质瘤中表达的临床意义及其生物学效应的探究
The clinical significance of miRNA-210 expression in human glioma and its biological effects
摘要
Abstract
Objective: To investigate the expression and clinical significance of microRNA-210 (miRNA-210) in glioma tissues and its effect on the proliferation and invasion of glioma cells.Methods: A total of 42 cases of brain glioma and 20 cases of benign meningioma were collected in the department of neurosurgery in our hospital.Quantitative Real-time PCR (qPCR) was used to detect the expression of miRNA-210 in tissue of patients, and Kaplan-Meier survival analyzed the relationship between the expression and the patients prognosis.Small interference RNA (siRNA) was used to interfere the expression of miRNA-210 in U251 human glioma cell line in vitro.MTT assay was used to detect the ability of cell proliferation.Transwell invasion assay was used to detect the ability of cell invasion.Results:: The expression of miRNA-210 in 29 cases of high grade gliomas was higher than that of 13 cases of low grade gliomas and 20 cases of benign meningioma (P<0.05).Kaplan-Meier survival showed the prognosis glioma patients with high expression of miRNA-210 was poorer than patients with low expression (P<0.05).SiRNA was transfected to U251 cells successfully, and the expression of miR-210 in U251 simiR-210 cell was decreased significantly compared to U251 control.The Results: of MTT showed the solution relative absorbance of U251 simiR-210 cells was significantly lower than that of U251 control cell in 72 h (P<0.05).The Results: of MTT assay showed the solution relative absorbance of U251 simiR-210 cells was significantly lower than that of U251 control cell in 72 h.The Results: of transwell invasion assay showed the cell number of penetrating membrane in U251 simiR-210 cells was significantly less than that of U251 in control cells.Conclusions: The expression of miRNA-210 is closely related to the malignant degree and prognosis of glioma patients, which can promote the proliferation and invasion of glioma cells.关键词
miRNA-210/人脑胶质瘤/增殖能力/侵袭能力Key words
miRNA-210/Glioma/Proliferation ability/Invasion ability分类
医药卫生引用本文复制引用
徐鹏翔,李强,许琼冠,张彩彩..miRNA-210在人脑胶质瘤中表达的临床意义及其生物学效应的探究[J].海南医学院学报,2017,23(12):1675-1678,4.基金项目
海南省自然科学基金(20158308)It is supported by Natural Science Foundation of Hainan Province (20158308). （20158308）
