海南医学院学报2017，Vol.23Issue(12)：1712-1715,4.DOI:10.13210/j.cnki.jhmu.20170627.013
超声剪切波速度与原发性肝癌病灶中癌基因、抑癌基因表达及血管新生因子含量的关系
Relationship of ultrasonic shear wave velocity with oncogene and tumor suppressor gene expression in primary liver cancer lesions as well as angiogenesis factor contents
摘要
Abstract
Objective: To discuss the relationship of ultrasonic shear wave velocity (SWV) with oncogene and tumor suppressor gene expression in primary liver cancer lesions as well as angiogenesis factor contents.Methods: A total of 100 patients with primary liver cancer who underwent surgical treatment in our hospital between March 2014 and September 2016 were collected as observation group, and 50 healthy subjects who received physical examination in our hospital during the same period were collected as normal control group.The ultrasonic SWV levels of two groups of subjects were measured before the operation, and the observation group were further divided into high SWV group and low SWV group, 50 cases in each group.Intraoperative tumor tissue samples were kept, and fluorescence quantitative PCR was used to determine the mRNA expression of oncogenes and tumor suppressor genes.Enzyme-linked immunosorbent assay was used to determine serum contents of angiogenesis factors in observation group before operation.Results:: Hepatic ultrasonic SWV level in observation group was significantly higher than that in normal control group (P<0.05);proto-oncogene CK, Ki67, Gly-3, Survivin and Pokemon mRNA expression in tumor tissue of high SWV group were higher than those of low SWV group while tumor suppressor genes Tg737, p16, p27, PTEN and runx3 mRNA expression were lower than those of low SWV group;serum angiogenesis factors VEGF, MMP-9 and IGF-1R contents were higher than those in low SWV group (P<0.05).Conclusions: The hepatic ultrasonic SWV level increases in patients with primary liver cancer, and the SWV level is directly correlated with oncogene and tumor suppressor gene expression as well as angiogenesis factor contents.关键词
原发性肝癌/超声剪切波速度/癌基因/抑癌基因/血管新生Key words
Primary liver cancer/Ultrasonic shear wave velocity/Oncogene/Tumor suppressor gene/Angiogenesis分类
医药卫生引用本文复制引用
尹星,何华,牛子长..超声剪切波速度与原发性肝癌病灶中癌基因、抑癌基因表达及血管新生因子含量的关系[J].海南医学院学报,2017,23(12):1712-1715,4.基金项目
国家自然科学基金资助(81170108)It was supported by National Natural Science Foundation of China (81170108). （81170108）
